Uncoated Human IL-37 ( Interleukin 37 ) ELISA Kit (E-UNEL-H0395)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-37 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-37 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IL-37 . You can calculate the concentration of IL-37 in the samples by comparing the OD of the samples to the standard curve.

IL-37,also known as IL-1F7,belongs to the IL-1 family.   There are five isoforms generated by alternative splicing,identified as IL-37a-e.   IL-37b is the largest isoform and IL-37a contains a unique N-terminal sequences,absent in other isoforms.   IL-37 is exclusively expressed only in human cells,but not found in murine cells.   IL-37 isoforms are expressed in a tissue-specific manner.   Its expression is upregulated upon LPS stimulation as well as TLR agonists in human blood monocytes and macrophages,whereas it is downregulated by IL-12,IL-32 and combination of GM-CSF and IL-4.   IL-37 has been shown to translocate to the nucleus upon LPS treatment.   IL-37 is a broad anti-inflammatory cytokine.     IL-37a and IL-37b inhibits LPS-treated pro-inflammatory cytokine production in M1-differentiated macrophages and dendritic cells via IL-18Rα and SIGIRR/IL-1R8.