Uncoated Human IL-5 (Interleukin 5) ELISA Kit (E-UNEL-H0267)

Name: Uncoated Human IL-5 (Interleukin 5) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-H0267
Price: 260.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Uncoated Human IL-5 (Interleukin 5) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 260.00	- +
	96T*15	$ 550.00	- +

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Human
Specificity	This kit recognizes Human IL-5 in samples.No significant cross-reactivity or interference between Human IL-5 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.373	2.353	2.363	2.279
500	1.596	1.582	1.589	1.505
250	0.773	0.809	0.791	0.707
125	0.428	0.446	0.437	0.353
62.5	0.272	0.284	0.278	0.194
31.25	0.186	0.174	0.180	0.096
15.63	0.136	0.126	0.131	0.047
0	0.082	0.086	0.084	-

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.