Uncoated Human PPY (Pancreatic polypeptide) ELISA Kit (E-UNEL-H0363)

Name: Uncoated Human PPY (Pancreatic polypeptide) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-H0363
Price: 260.0 USD
Availability: InStock

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Uncoated Human PPY (Pancreatic polypeptide) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 260.00	- +
	96T*15	$ 550.00	- +

Ancillary Products Available: Ancillary Reagent Kit

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Human
Specificity	This kit recognizes Human PPY in samples.No significant cross-reactivity or interference between Human PPY and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	2.285	2.287	2.286	2.208
2000	1.439	1.493	1.466	1.388
1000	0.796	0.758	0.777	0.699
500	0.493	0.479	0.486	0.408
250	0.266	0.270	0.268	0.19
125	0.175	0.163	0.169	0.091
62.5	0.128	0.124	0.126	0.048
0	0.082	0.074	0.078	-

Background

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.