Uncoated Human SIRT1 ( Sirtuin 1 ) ELISA Kit (E-UNEL-H0391)

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	96T*5	$ 399.00
	96T*15	$ 958.00

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Sensitivity	0.06ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	24h
Reacitivity	Human
Specificity	This kit recognizes SIRT1 in samples.No significant cross-reactivity or interference between SIRT1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SIRT1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SIRT1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SIRT1 . You can calculate the concentration of SIRT1 in the samples by comparing the OD of the samples to the standard curve.

Background

SIRT1,the human homolog of the S. cerevisiae Sir2 protein,functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage,SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuates p53-mediated functions. When overexpressed in mouse embryo fibroblasts,SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells,SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.

Gene Alias	Sirt1
Gene ID	23411
Uniport ID	Q96EB6
Protein Alias	Sirt1
Research Area	Cell Biology , Signal Transduction

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