Uncoated Human TSP-4 ( Thrombospondin-4 ) ELISA Kit (E-UNEL-H0393)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSP-4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TSP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSP-4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TSP-4 . You can calculate the concentration of TSP-4 in the samples by comparing the OD of the samples to the standard curve.

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation,migration,adhesion and attachment,inflammatory response to CNS injury,regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling.  Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage,contributing to cardioprotective and adaptive ECM remodeling.  Plays a role in ER stress response,via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload.  May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury.  May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.