Uncoated Human VCAN ( Versican/PG-M ) ELISA Kit (E-UNEL-H0394)
Product Summary
The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
| Sensitivity | 0.13ng/mL |
| Detection Range | 0.63-40 ng/mL |
| Sample Volume | 100 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 24h |
| Reactivity | Human |
| Specificity | This kit recognizes VCAN in samples.No significant cross-reactivity or interference between VCAN and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T*5 / 96T*15 |
| Storage | -20℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VCAN . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VCAN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VCAN , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VCAN . You can calculate the concentration of VCAN in the samples by comparing the OD of the samples to the standard curve.
Background
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility,growth and differentiation. Binds hyaluronic acid.
| Gene Alias | VCAN |
| Gene ID | 1462 |
| Uniport ID | P13611 |
| Protein Alias | VCAN |
| Research Area | Signal Transduction |
| Cat.No. | Product Name | Clone No. |
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