Uncoated Mouse ALPL ( Alkaline Phosphatase,Liver/Bone/Kidney ) ELISA Kit (E-UNEL-M0164)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ALPL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ALPL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ALPL , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ALPL . You can calculate the concentration of ALPL in the samples by comparing the OD of the samples to the standard curve.

Alkaline phosphatases function physiologically as homodimers.  They catalyze the hydrolysis of phosphomonoesters and release inorganic phosphate.  In humans,four isoenzymes have been described: a tissue-nonspecific (TNAP) and three tissue-specific placental (PLAP),intestinal (IAP),and germ cell (GCAP).  TNAP is a cell membrane-bound ecto-enzyme that releases inorganic phosphate from phosphate-enriched substrates.  Also,ALPL induces the hydrolysis of extracellular inorganic pyrophosphate (PPi) which is a potent endogenous inhibitor of calcification both in vivo and in vitro.  Increased activity of the enzyme alkaline phosphatase is associated with lower levels of PPi and vascular calcification present in patients with chronic kidney disease.