Uncoated Mouse CD27(CD27 antigen) ELISA Kit (E-UNEL-M0189)
Product Summary
The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
| Detection Range | 0.78-50 ng/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 24 h |
| Reactivity | Mouse |
| Specificity | This kit recognizes Mouse CD27 in samples.No significant cross-reactivity or interference between Mouse CD27 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T*5 / 96T*15 |
| Storage | -20℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD27. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD27. You can calculate the concentration of Human CD27 in the samples by comparing the OD of the samples to the standard curve.
Background
| Uniport ID | P41272 |
| Research Area | Cancer , Immunology , Stem Cells |
| Cat.No. | Product Name | Clone No. |
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