Uncoated Mouse FAS/CD95 ( Factor Related Apoptosis ) ELISA Kit (E-UNEL-M0158)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FAS/CD95 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FAS/CD95 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FAS/CD95 . You can calculate the concentration of FAS/CD95 in the samples by comparing the OD of the samples to the standard curve.

CD95 is a 45 kD single chain type I glycoprotein also known as Fas,APO-1,and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes,monocytes,neutrophils,and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.