Uncoated Mouse GAL3 ( Galectin 3 ) ELISA Kit (E-UNEL-M0157)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAL3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAL3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GAL3 . You can calculate the concentration of GAL3 in the samples by comparing the OD of the samples to the standard curve.

Galectin-3 is a member of the lectin family,which includes 15 mammalian lectin members.    Like all galectins,galectin-3 contains a carbohydrate recognition binding domain of about 130 amino acids that enables the specific binding of β-galactosides.    This protein has been shown to be involved in cell adhesion,cell activation,chemoattraction,cell growth and differentiation,cell cycle,and apoptosis.    Galectin-3 induces pro-inflammatory cytokines and inhibits Th2 type cytokine production.    It chemoattracts monocytes and macrophages,and activates and degranulates basophils and mast cells.    Galectin-3 has been shown to be involved in cancer,inflammation,fibrosis,heart disease,and stroke.    It has also been shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure,including myofibroblast proliferation,tissue repair,inflammation,fibrogenesis,and ventricular remodeling.    In addition,galectin-3 was found to contribute to tumor cell evasion of the immune system.    Galectin-3 is increasingly being used as a diagnostic marker for different cancers.    It can be screened for and used as a prognostic factor to predict the progression of the cancer.