Uncoated Mouse IL-18 (Interleukin 18) ELISA Kit (E-UNEL-M0115)

Name: Uncoated Mouse IL-18 (Interleukin 18) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-M0115
Price: 260.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Uncoated Mouse IL-18 (Interleukin 18) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 260.00	- +
	96T*15	$ 550.00	- +

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Mouse
Specificity	This kit recognizes Mouse IL-18 in samples.No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.305	2.385	2.345	2.261
500	1.613	1.555	1.584	1.5
250	0.802	0.770	0.786	0.702
125	0.427	0.437	0.432	0.348
62.5	0.284	0.272	0.278	0.194
31.25	0.183	0.175	0.179	0.095
15.63	0.132	0.134	0.133	0.049
0	0.082	0.086	0.084	-

Background

Interleukin-18 (L-18) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.