Uncoated Mouse OxLDL ( Oxidized Low Density Lipoprotein ) ELISA Kit (E-UNEL-M0165)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OxLDL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OxLDL , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of OxLDL . You can calculate the concentration of OxLDL in the samples by comparing the OD of the samples to the standard curve.

Receptor that mediates the recognition,internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells.  OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction,resulting in pro-inflammatory responses,pro-oxidative conditions and apoptosis.  Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release,monocyte adhesion and apoptosis.  In addition to binding oxLDL,it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells,thereby participating in cell-mediated antigen cross-presentation.  Also involved in inflammatory process,by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation.  Also acts as a receptor for advanced glycation end (AGE) products,activated platelets,monocytes,apoptotic cells and both Gram-negative and Gram-positive bacteria.