Uncoated Porcine IL-1β (Interleukin 1 Beta) ELISA Kit (E-UNEL-P0007)

Name: Uncoated Porcine IL-1β (Interleukin 1 Beta) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-P0007
Price: 399.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Uncoated Porcine IL-1β (Interleukin 1 Beta) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 399.00	- +
	96T*15	$ 958.00	- +

Ancillary Products Available: Ancillary Reagent Kit

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Porcine
Specificity	This kit recognizes Porcine IL-1β in samples.No significant cross-reactivity or interference between Porcine IL-1β and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	2.338	2.266	2.302	2.22
250	1.577	1.551	1.564	1.482
125	1.008	1.004	1.006	0.924
62.5	0.581	0.559	0.570	0.488
31.25	0.313	0.321	0.317	0.235
15.63	0.179	0.191	0.185	0.103
7.81	0.132	0.144	0.138	0.056
0	0.082	0.082	0.082	-

Background

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen; induces prostaglandin synthesis; neutrophil influx and activation; T-cell activation and cytokine production; B-cell activation and antibody production; and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.