Uncoated Rat sCD14 ( Soluble Cluster of Differentiation 14 ) ELISA Kit (E-UNEL-R0088)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sCD14 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for sCD14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain sCD14 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of sCD14 . You can calculate the concentration of sCD14 in the samples by comparing the OD of the samples to the standard curve.

CD14 is a pattern recognition receptor important in innate immunity.    CD14 exists in two forms,as a glycosylphosphatidylinositol-anchored protein on the surface of mononuclear cells and as a soluble protein in the blood.    CD14 is shed from the surface of CD14-expressing cells,resulting in the soluble CD14 protein (sCD14).    CD14 is down regulated from the cell membrane on stimulated monocytes by different factors such as LPS,IFNγ,PMA,calcium ionophore A 23187,and antibodies against CD14.    CD14 can be released from the cells with GPI-phospholipase C and D (PLC/D).    Membrane associated CD14 is a pro-inflammatory co-receptor for LPS,a characteristic constituent of the cell wall of Gram-negative bacteria.   Elevated levels of sCD14 has been detected in serum of patients with different diseases such as systemic lupus erythematosus,HIV infected patients,common variable immunodeficiency (CVID),and nonalcoholic fatty liver disease (NAFLD).    Also,high levels of sCD14 have been associated with infectious diseases,including sepsis.