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Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method) (E-BC-K329-S)

All Size Price Qty
100Assays $ 150.00
50Assays $ 120.00
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For research use only.

Detection Principle In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:
Synonyms BUN
Sample Type Serum,Plasma,Saliva,Urine
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (520 nm)
Research Area Liver And Renal Function
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.12 mmol/L
Detection Range 0.12-15 mmol/L
Precision inter-assay CV: 9.9% | intra-assay CV: 4.9%
Assay Time 1 h
Cat.No. Product Name Sizes
E-BC-K183-S Urea (BUN) Colorimetric Assay Kit (Urease Method) 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human urine 45-60
Human plasma 1
Human serum 1
Human saliva 1
Mouse serum 1
Rat urine 45-60
Rabbit plasma 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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