Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method) (E-BC-K329-S)
For research use only.
| Detection Principle | In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows: |
| Synonyms | BUN |
| Sample Type | Serum,Plasma,Saliva,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (520 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.12 mmol/L |
| Detection Range | 0.12-15 mmol/L |
| Precision | inter-assay CV: 9.9% | intra-assay CV: 4.9% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human urine | 45-60 |
| Human plasma | 1 |
| Human serum | 1 |
| Human saliva | 1 |
| Mouse serum | 1 |
| Rat urine | 45-60 |
| Rabbit plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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