Uric Acid (UA) Colorimetric Assay Kit (E-BC-K016-S)
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For research use only.
| Detection Principle | Uric acid (UA) in the protein-free filtrate reduces phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide. The intensity of the blue color is proportional to the uric acid concentration. The uric acid content can be calculated by measuring the OD value at 690 nm. |
| Synonyms | UA |
| Sample Type | Serum,Plasma,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (690 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.58 mg/L |
| Detection Range | 0.58-100 mg/L |
| Precision | inter-assay CV: 2.6% | intra-assay CV: 1.8% |
| Sample Volume | 200 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human urine | 8-10 |
| Human serum | 1 |
| Dog serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1-2 |
| Porcine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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