Uric Acid (UA) Fluorometric Assay Kit (E-BC-F018)
For research use only.
| Detection Principle | Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated. |
| Synonyms | UA |
| Sample Type | Serum,Plasma,Urine,Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.03 μmol/L |
| Detection Range | 0.03-15 μmol/L |
| Precision | inter-assay CV: 7.2% | intra-assay CV: 1.5% |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10-20 |
| Human urine | 80-100 |
| Human hydrothorax | 50-60 |
| Rat urine | 10-20 |
| Rabbit serum | 5-10 |
| Rat serum | 10-20 |
| Porcine serum | 1 |
| 10% Rat liver tissue homogenate | 10-20 |
| 10% Rat kidney tissue homogenate | 30-40 |
| 10% Rat lung tissue homogenate | 10-20 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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