Uricase Acitivity Colorimetric Assay Kit (E-BC-K896-M)
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For research use only.
| Detection Principle |
Uricase is an enzyme in the purine decomposition pathway, which can specifically catalyze the oxidation of uric acid into allantoin with higher solubility, thereby reducing the blood uric acid level. The detection principle of this kit is: uricase can oxidize the substrate, and the substrate has the maximum absorption peak at 293 nm. The uricase activity was calculated by measuring the OD value reduction at 293 nm. |
| Sample Type | Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (optimum wavelength: 293 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 U/L |
| Detection Range | 0.06-4.00 U/L |
| Precision | inter-assay CV: 6.7-8.8% | intra-assay CV: 2.6-3.4% |
| Sample Volume | 40 μL (tissue homogenate) |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1-3 |
| 10% Porcine liver tissue homogenate | 1-3 |
| 10% Spinach tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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