Flow Cytometry
Flow Cytometry FAQs
In order to provide better customer support and address customers' questions about FCM antibodies, we have compiled and published answers to the frequently-asked questions (FAQs) about FCM antibodies, which will be continuously updated.
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Theoretically possible, but separate permeabilization buffer are recommended for optimal results: Nuclear membrane permeabilization buffer (stronger, ideal for nuclear targets like FOXP3) may damage cell membrane epitopes. Cell membrane permeabilization buffer (milder, better for cytoplasmic/membrane targets like cytokines).
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Antibody isotypes/subclasses are categorized based on the heavy chain structure of immunoglobulins (Ig). In mammals: 5 main Ig classes: IgA (α), IgD (δ), IgE (ε), IgG (γ), and IgM (μ). Human IgG subclasses: IgG1, IgG2, IgG3, IgG4. Mouse IgG subclasses: IgG1, IgG2a, IgG2b, IgG3. In flow cytometry: IgG subclasses (e.g., anti-CD3, CD4, CD8 for T cells; CD19, CD20 for B cells) are commonly used to detect surface antigens.
