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3-NT(3-Nitrotyrosine) ELISA Kit

  • Cat.No.:E-EL-0040

  • Reactivity: Universal

To Purchase E-EL-0040

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes 3-NT in samples. No significant cross-reactivity or interference between 3-NT and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 3-NT. During the reaction, 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 3-NT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 3-NT were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 5.00 12.50 38.30 5.10 13.10 36.60
Standard deviation 0.30 0.70 1.80 0.30 0.60 1.90
CV (%) 6.00 5.60 4.70 5.88 4.58 5.19


The recovery of 3-NT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-108 102
EDTA plasma(n=8) 91-108 99
Cell culture media(n=8) 93-105 100


Samples were spiked with high concentrations of 3-NT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms3-Nitro-L-Tyrosine, Nitrotyrosine
Research AreaSignal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF- κ B Activation and iNOS Protein Expression

    DOI: 10.1155/2020/7106525

    PMID: 32148655

    Sample: Tissue homogenate
  2. Archives of Biochemistry and Biophysics (2019) IF: 3.118
    Effect of a high sucrose and high fat diet in BDNF (+/-) mice on oxidative stress markers in adipose tissues

    DOI: 10.1016/

    PMID: 30797748

    Sample: Serum
  3. Andrology (2020) IF: 2.86
    Low androgen status inhibits erectile function by inducing eNOS uncoupling in rat corpus cavernosum

    DOI: 10.1111/andr.12844

    PMID: 32594586

    Sample: Tissue homogenate
  4. Molecular vision (2018) IF: 2.219
    Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress

    PMID: 30090013

    Sample: Tissue homogenate
  5. Biological trace element research (2018) IF: 2.361
    Melatonin Ameliorates Neuropharmacological and Neurobiochemical Alterations Induced by Subchronic Exposure to Arsenic in Wistar Rats

    DOI: 10.1007/s12011-018-1537-1

    PMID: 30306420

    Sample: Tissue homogenate
  6. Plos One (2017) IF: 2.806
    Reduction Of Oxidative-Nitrosative Stress Underlies Anticataract Effect Of Topically Applied Tocotrienol In Streptozotocin-Induced Diabetic Rats.

    DOI: 10.1371/journal.pone.0174542

    PMID: 28350848

    Sample: Tissue homogenate
  7. Nigerian journal of clinical practice (2019) IF: 0.713
    Markers of lipid and protein peroxidation among Nigerian university students with dysmenorrhea

    DOI: 10.4103/njcp.njcp_279_18

    PMID: 30729939

    Sample: Plasma
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