Current Position:Home>>Kits>>ELISA Kits>> A-CoA(Acetyl Coenzyme A) ELISA Kit
Cat.No.:E-EL-0125
Welcome to order from local distributors.
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.19 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes A-CoA in samples. No significant cross-reactivity or interference between A-CoA and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of A-CoA concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level A-CoA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level A-CoA were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 1.11 | 2.68 | 7.94 | 1.00 | 2.48 | 7.46 |
Standard deviation | 0.08 | 0.12 | 0.40 | 0.07 | 0.11 | 0.27 |
CV (%) | 7.21 | 4.48 | 5.04 | 7.00 | 4.44 | 3.62 |
The recovery of A-CoA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-99 | 94 |
EDTA plasma (n=8) | 86-97 | 91 |
Cell culture media (n=8) | 93-108 | 99 |
Research Area | Tags and Cell Markers, Cardiovascular, Metabolism |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
|
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
|
3. Aspirate and wash the plate for 3 times |
|
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
|
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
|
6. Add 50μL Stop Solution |
|
7. Read the plate at 450nm immediately. Calculation of the results |
Sample: B16-F10 cell,CT-26 cell |
PMID: 35775648 |
Sample: Huh7 cell,Hep3B cell,MHCC-97H cell,HepG2 cell |
DOI: 10.1111/jipb.13283 |
|
PMID: 35603832 |
Sample: leaf,seedling |
PMID: 35917871 |
Sample: liver |
PMID: 36145507 |
Sample: AGS cell |
Sample: Liver tissue |
DOI: 10.1155/2020/5849794 |
|
Sample: Tissue homogenate |
Sample: liver tissue |
Sample: liver,primary hepatocytes |
PMID: 36404863 |
Sample: plasma |
W********hSubmitted [ Mar 07 2023 ]
Asked: hi. I am a researcher working at the institute for basic science, South Korea. We purchased your kit (i.e. Acetyl CoA elisa kit) this time and are trying to utilize it, but strangely, there is a reaction with your reference/standard sample, but there is no reaction with the Acetyl CoA we have (Roche and Sigma-aldrich, two products). We are wondering if your product is actually capable of accurately measuring acetyl CoA and would like to get some advice from your technical team. We look forward to your response. Best regards
Reply
adminSubmitted [ Mar 07 2023 ]
Answered: Dear Wuhyun Koh, Thanks for your feedback. At present, we can guarantee the applicability and detection rate of the E-EL-0125 for natural samples only. We have verified a large number of serum, plasma and some tissue samples, and the detection effect conforms to the literature report very well. We have not done a lot of validation on some recombinant proteins and other products so far, and there may indeed be undetectable situations. We have searched Merck's official website for A-CoA products. Sigma Aldrich contains A-CoA-3Na (Cat No.: A2056) and A-CoA-Li+(Cat No.: A2181), and Roche products (SKU: 10101893001; 10101907001; 11585371001) contain A-CoA-Li3 according to the chemical composition description on the product page. We speculate that there may be some difference between those product above and composition of our standard in A-CoA ELISA kit, which lead to the the possibility of undetected. A-coa is a chemical (Acetyl-CoA C23H38N7O17P3S), and it's a small molecule, which is not applicable the spatial structure/label commonly used for conventional large molecules In addition, different manufacturers have different methods for A-CoA synthesis, and the reagents or other factors added in the process of may have some influence on reaction system of ELISA, thus affecting the detected OD value. Based the above situation, we recommend you to use natural samples to detect. Thanks for your kind understanding. If any further questions, pls feel free to contact customer00@elabscience.com Best regards, Crystal