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ADMA(Asymmetrical Dimethylarginine) ELISA Kit

  • Cat.No.:E-EL-0042

  • Reactivity: Universal

To Purchase E-EL-0042

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 ng/mL
Sensitivity 9.38 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes ADMA in samples. No significant cross-reactivity or interference between ADMA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of ADMA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADMA. During the reaction, ADMA in the sample or standard competes with a fixed amount of ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ADMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ADMA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ADMA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 53.20 120.80 371.60 50.50 121.30 371.70
Standard deviation 3.70 6.30 19.30 3.40 5.90 19.70
CV (%) 6.95 5.22 5.19 6.73 4.86 5.30


The recovery of ADMA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-102 94
EDTA plasma(n=8) 96-111 102
Cell culture media(n=8) 94-108 101


Samples were spiked with high concentrations of ADMA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsN, N-Dimethylarginine, NG-Dimethylarginine dihydrochloride, 2-Amino-5-[(amino-dimethylaminomethylene)amino]pentanoic acid
Research AreaSignal transduction, Cardiovascular

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


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View more details about our Publication Reward >>

  1. GeroScience (2021) IF: 7.713
    Early manifestation of aging-related vascular dysfunction in human penile vasculature—A potential explanation for the role of erectile dysfunction as a harbinger of systemic vascular disease

    DOI: 10.1007/s11357-021-00507-x

    Sample: plasma
  2. Antioxidants (2021) IF: 6.313
    Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature

    DOI: 10.3390/antiox10050722

    PMID: 34064366

    Sample: Serum
  3. Frontiers in Medicine (2022) IF: 5.058
    Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.

    DOI: 10.3389/fmed.2022.953749

    Sample: plasma
  4. Metabolites (2020) IF: 4.097
    Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma

    DOI: 10.3390/metabo11010010

    PMID: 33375435

    Sample: Serum
    Relationship between asymmetric dimethylarginine, nitrite and genetic polymorphisms: Impact on erectile dysfunction therapy

    DOI: 10.1016/j.niox.2017.10.006

    Sample: Plasma
  6. Journal of Clinical Medicine (2021) IF: 4.242
    Cardiovascular Risk Factors and Their Relationship with Vascular Dysfunction in South African Children of African Ancestry

    DOI: 10.3390/jcm10020354

    PMID: 33477761

    Sample: Urine
    Dihydromyricetin increases endothelial nitric oxide production and inhibits atherosclerosis through microRNA‐21 in apolipoprotein E‐deficient mice

    DOI: 10.1111/jcmm.15278

    Sample: plasma
    Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

    DOI: 10.1016/j.ejphar.2019.172786

    PMID: 31712060

    Sample: Serum
    The association between dysregulated adipocytokines in early pregnancy and development of gestational diabetes

    DOI: 10.1002/dmrr.2926

    Sample: Serum
    DDAH1 and DDAH2 polymorphisms associate with asymmetrical dimethylarginine plasma levels in erectile dysfunction patients but not in healthy controls

    DOI: 10.1016/j.niox.2019.08.001

    PMID: 31394201

    Sample: Plasma
  11. Obesity Research & Clinical Practice (2021) IF: 2.288
    Fermented soybean enhances post-meal response in appetite-regulating hormones among Indonesian girls with obesity

    DOI: 10.1016/j.orcp.2021.06.005

    PMID: 34147377

    Sample: plasma
  12. Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
    Comparison between the Effects of Bretschneider’s HTK Solution and Cold Blood Cardioplegia on Systemic Endothelial Functions in Patients who Undergo Coronary Artery Bypass Surgery: a Prospective Randomized and Controlled Trial

    DOI: 10.21470/1678-9741-2019-0327

    Sample: Serum
    Comparison of asymmetric dimethylarginine levels between pre- and post-menopausal women - a cross-sectional study from Rawalpindi.

    DOI: 10.5455/jpma.296507

    Sample: Serum
  14. African Health Sciences (2021) IF: 0.927
    Gut permeability is associated with hypertension and measures of obesity but not with Endothelial Dysfunction in South African youth

    DOI: 10.4314/ahs.v21i3.26

    Sample: plasma
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