×

ADMA(Asymmetrical Dimethylarginine) ELISA Kit

  • Cat.No.:E-EL-0042

  • Reactivity: Universal

To Purchase E-EL-0042

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 ng/mL
Sensitivity 9.38 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes ADMA in samples. No significant cross-reactivity or interference between ADMA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of ADMA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADMA. During the reaction, ADMA in the sample or standard competes with a fixed amount of ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ADMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ADMA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ADMA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 53.20 120.80 371.60 50.50 121.30 371.70
Standard deviation 3.70 6.30 19.30 3.40 5.90 19.70
CV (%) 6.95 5.22 5.19 6.73 4.86 5.30

Recovery

The recovery of ADMA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-102 94
EDTA plasma(n=8) 96-111 102
Cell culture media(n=8) 94-108 101

Linearity

Samples were spiked with high concentrations of ADMA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsN, N-Dimethylarginine, NG-Dimethylarginine dihydrochloride, 2-Amino-5-[(amino-dimethylaminomethylene)amino]pentanoic acid

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nitric Oxide-Biology And Chemistry (2017) IF: 4.181
    Relationship Between Asymmetric Dimethylarginine, Nitrite And Genetic Polymorphisms: Impact On Erectile Dysfunction Therapy.

    DOI: 10.1016/j.niox.2017.10.006

    PMID: 29074293

    Sample: Plasma
  2. European Journal of Pharmacology (2019) IF: 3.263
    Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

    DOI: 10.1016/j.ejphar.2019.172786

    PMID: 31712060

    Sample: Serum
  3. Nitric Oxide (2019) IF: 3.311
    DDAH1 and DDAH2 polymorphisms associate with asymmetrical dimethylarginine plasma levels in erectile dysfunction patients but not in healthy controls

    DOI: 10.1016/j.niox.2019.08.001

    PMID: 31394201

    Sample: Plasma
  4. Diabetes-Metabolism Research And Reviews (2017) IF: 3.263
    The Association Between Dysregulated Adipocytokines In Early Pregnancy And Development Of Gestational Diabetes.

    DOI: 10.1002/dmrr.2926

    PMID: 28806491

    Sample: Serum
  5. Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
    Comparison between the Effects of Bretschneider's HTK Solution and Cold Blood Cardioplegia on Systemic Endothelial Functions in Patients who Undergo Coronary Artery Bypass Surgery: a Prospective Randomized and Controlled Trial

    DOI: 10.21470/1678-9741-2019-0327

    PMID: 99999999

    Sample: Serum
  6. The Journal of the Pakistan Medical Association (2019) IF: 0.573
    Comparison of asymmetric dimethylarginine levels between pre- and post-menopausal women - a cross-sectional study from Rawalpindi

    DOI: 10.5455/JPMA.296507

    PMID: 31853108

    Sample: Serum
  7. INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY (2018)
    DIFFERENCES OF ASYMMETRIC DIMETHYL ARGININE LEVEL IN PATIENTS WITH DIABETIC NEPHROPATHY AND NON-DIABETIC NEPHROPATHY

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
How do you know
Elabscience:

*Verification code: