ADMA(Asymmetrical Dimethylarginine) ELISA Kit (E-EL-0042)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ADMA. During the reaction, Universal ADMA in the sample or standard competes with a fixed amount of Universal ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ADMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ADMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

N-g, N-G-dimethylarginine dihydrochloride is a methylated arginine derivative produced by the liver. It is associated with cirrhosis, alcoholic hepatitis, and acute liver failure (ALF). It has been used to: investigate its effect on cardiovascular activity in the ventrolateral medulla of the head (RVLM) in rats as a standard for measuring L-arginine and dimethylarginine.