AFM1(Aflatoxin M1) ELISA Kit

    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
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    >
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience

      Catalog number:E-TO-E018

      Size:
      • 96T
      Qty:
      - +
      Price: $300

      sample_type: Milk,Milk powder,Yogurt,Cheese

      Sensitivity: 0.01 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle


      This kit uses Competitive-ELISA as the method. It can detect Aflatoxin M1 (AFM1) in samples, such as raw milk, pure milk, reconstituted milk, and yogurt. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the reaction, AFM1 in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AFM1 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of AFM1. The concentration of AFM1 in the samples can be calculated by comparing the OD of the samples to the standard curve.


       

      Technical indicator

      Sensitivity: 0.01 ppb (ng/mL)

      Reaction mode: 2560min30min15min

      Detection limit: Raw milk, pure milk, reconstituted milk, yogurt---0.025ppb; Cheese—0.25ppb

      Cross-reactivity: Aflatoxin M1 ---100%, Aflatoxin M2 ---< 1%, Aflatoxin B1---40%,

                                   Aflatoxin B2---3%, Aflatoxin G1---20%, Aflatoxin G2---2%.

      Sample recovery rate: 100%±30%.

       

      Kits components


      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1.5mL each

      (0ppb, 0.01ppb, 0.03ppb, 0.09ppb, 0.27ppb)

      HRP Conjugate

      12ml

      Antibody Working Solution

      7mL

       Yogurt Sample Dilution

      10ml

      Sample Dilution

      45mL

      Substrate Reagent A

      7mL

      Substrate Reagent B

      7mL

      Stop Solution

      7mL

      20×Concentrated Wash Dilution

      25mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


       

      Other materials required but not supplied

      Instruments: Microplate reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g).

      Micropipettor: Single-channel (20-200 μL, 100-1000 μL).

      Reagents:  Methanol


      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.



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