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ALD(Aldosterone) ELISA Kit

  • Cat.No.:E-EL-0070

  • Reactivity: Universal

To Purchase E-EL-0070

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes ALD in samples. No significant cross-reactivity or interference between ALD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of ALD concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ALD. During the reaction, ALD in the sample or standard competes with a fixed amount of ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ALD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ALD in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ALD were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ALD were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.80 89.60 476.90 46.80 94.20 479.40
Standard deviation 2.70 4.30 20.00 3.20 5.70 23.00
CV (%) 5.21 4.80 4.19 6.84 6.05 4.80

Recovery

The recovery of ALD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-98 91
EDTA plasma(n=8) 96-112 102
Cell culture media(n=8) 90-102 96

Linearity

Samples were spiked with high concentrations of ALD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsALD

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Hypertension (2019) IF: 7.713
    Maternal High-Fructose Intake Induces Multigenerational Activation of the Renin-Angiotensin-Aldosterone System

    DOI: 10.1161/HYPERTENSIONAHA.119.12941

    PMID: 31327271

    Sample: Serum
  2. Redox Biology (2017) IF: 6.337
    Caveolin 1-Related Autophagy Initiated By Aldosterone-Induced Oxidation Promotes Liver Sinusoidal Endothelial Cells Defenestration.

    DOI: 10.1016/j.redox.2017.07.011

    PMID: 28734243

    Sample: Serum
  3. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Ameliorative Effects of Bredemolic Acid on Markers Associated with Renal Dysfunction in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2978340

    PMID: 32655765

    Sample: Plasma,Urine,Tissue homogenate
  4. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Amelioration of risk factors associated with diabetic nephropathy in diet-induced pre-diabetic rats by an uracil-derived diimine ruthenium(II) compound

    DOI: 10.1016/j.biopha.2020.110483

    PMID: 32768965

    Sample: Plasma,Tissue homogenate
  5. Frontiers In Cellular And Infection Microbiology (2017) IF: 4.3
    Microbiota Modulate Anxiety-Like Behavior And Endocrine Abnormalities In Hypothalamic-Pituitary-Adrenal Axis.

    DOI: 10.3389/fcimb.2017.00489

    PMID: 29250490

    Sample: Tissue homogenate
  6. Molecules (2017) IF: 3.098
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  7. Journal of Pharmacy and Pharmacology (2019) IF: 2.571
    The effect of eplerenone on the renin-angiotensin-aldosterone system of rats with thyroid dysfunction

    DOI: 10.1111/jphp.13168

    PMID: 31579950

    Sample: Serum
  8. Drug and chemical toxicology (2018) IF: 2.405
    Nicotine exposure suppresses hyperinsulinemia and improves endothelial dysfunction mediators independent of corticosteroids in insulin-resistant oral contraceptive-treated female rats

    DOI: 10.1080/01480545.2017.1413109

    PMID: 29268635

    Sample: Plasma
  9. Canadian journal of physiology and pharmacology (2018) IF: 2.21
    Ameliorative effect of low-dose spironolactone on obesity and insulin resistance is through replenishment of estrogen in ovariectomized rats

    DOI: 10.1139/cjpp-2018-0416

    PMID: 30422674

    Sample: Plasma
  10. PloS one (2018) IF: 2.766
    Neuroglobin correlates with cryptochrome-1 in obstructive sleep apnea with primary aldosteronism

    DOI: 10.1371/journal.pone.0204390

    PMID: 30235329

    Sample: Serum
  11. Journal of Diabetes Research (2018) IF: 3.04
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.

    DOI: 10.1155/2018/7341242

    PMID: 30009183

    Sample: Tissue homogenate
  12. Molecules (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  13. Archives of physiology and biochemistry (2018) IF: 1.806
    Blockade of mineralocorticoid receptor ameliorates oral contraceptive-induced insulin resistance by suppressing elevated uric acid and glycogen synthase kinase-3 instead of circulating mineralocorticoid

    DOI: 10.1080/13813455.2018.1509220

    PMID: 30318954

    Sample: Plasma
  14. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology (2020)
    Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome

    DOI: 10.4196/kjpp.2020.24.4.319

    PMID: 32587126

    Sample: Serum
  15. Chinese Herbal Medicines (2019)
    Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats

    DOI: 10.1016/j.chmed.2018.12.004

    PMID: 99999999

    Sample: Plasma,Serum,Tissue homogenate
  16. Bangladesh Journal of Pharmacology (2018) IF: 0.814999999
    Mechanism of the diuretic activity of Descurainia sophia seed.

    DOI: 10.3329/bjp.v13i2.35931

    Sample: Urine
  17. Journal of Diseases and Medicinal Plants (2018) IF: /
    Transdermal Drug Delivery of Asiatic Acid Influences Renal Function and Electrolyte Handling in Plasmodium berghei-Infected Sprague-Dawley Male Rats.

    DOI: 10.11648/j.jdmp.20180401.13

    Sample: Plasma
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