AMO(Amoxicillin) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E077

      Size:
      • 96T
      Qty:
      - +
      Price: $370

      sample_type: Tissue,Milk,Egg,Duck

      Sensitivity: 0.03 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Amoxicillin (AMO) in samples, such as tissues, eggs, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, AMO in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AMO antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of AMO. The concentration of AMO in the samples can be calculated by comparing the OD of the samples to the standard curve.



      Technical indicator

      Sensitivity:  0.03 ppb (ng/mL)

      Reaction mode:  25℃, 30 min~30min~20min

      Detection limit:  Raw milk---0.2 ppb; Tissue, Eggs ---1 ppb; Duck---1.5 ppb

      Cross-reactivity:

      Amoxicillin

      100%

      Penicillin

      16%

      Ampicillin

      9%

      Nafcillin

      2.4%

      Oxacillin

      7.1%

      Cloxacillin

      6.3%

      Dicloxacillin

      11.8%

      Azlocillin

      14.7%

      Methicillin

      5.2%

      Sample recovery rate: Raw milk, Eggs, Tissue---90%±30%.


      Kits components

      Item

      Specifications

      ELISA Microplate

      96 wells

      Standard Liquid

      1.5 mL each

      (0 ppb, 0.03 ppb, 0.09 ppb, 0.27 ppb, 0.81 ppb, 2.43 ppb)

      Antibody working Solution

      7 mL

      HRP Conjugate

      12 mL

      Concentrated Sample Solution 1

      40 mL

      50×Concentrated Sample Solution 2

      10 mL

      Substrate Reagent A

      7 mL

      Substrate Reagent B

      7 mL

      Stop Solution

      7 mL

      20×Concentrated Wash Buffer

      25 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other materials required but not supplied

      Instrument: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01g), 

      High-precision transferpettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL) 

      Reagents: ZnSO4·7H2O


      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8℃, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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