Annexin V-APC/7-AAD Apoptosis Kit
Price: $ 398
Price: $ 260
Price: $ 150
Price: $ 75
- Kit Type: Apoptosis/Annexin V
For research use only. Order now, ship in 3 days
Detection Principle | Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished. |
Detection Method | Fluorometric Method;Flow Cytometry |
Sample Type | Cell samples |
Assay Time | 40min |
Detection Instrument | Flow Cytometer |
Dye Type | APC |
Ex/Em | 650/660 |
Channel Set | APC |
Other Reagents Required | PBS(E-BC-R187), Deionized water |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Expiration date | 12 months |
Shipping | Ice bag |
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Q1:How to set the gate for rathippocampal neuron apoptosis assay?
Annexin V assay is normally used for cell line samples, thus only debris need to be excluded by gating. Since there aremultiple types of cells in tissue samples, it depends on the purpose of experiment, whether it means to detect apoptosis of the whole tissue sample or some specific cells. Cells can be gated by FSC/SSC or some other biomarkers. Besides, operation of tissue sample preparation may also effect the result, Inappropriate operation may also result in false positives due to sample handling.
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Q2:Annexin V Kit can be used to detect plant samples?
There is no such experience as verification yet. It is recommanded to take a pre-test if necessary.
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Q3:Can PBS be used to replace Binding Buffer in Annexin V assay?
No, because Annexin V binds to phosphatidylserine (PS) dependent on Ca2+, which is not present in common PBS solutions, while Binding buffers contain Ca2+ to facilitate Annexin V binding to PS. Without Binding Buffer, Annexin V binding capacity will be reduced, resulting in significantly reduced positive signal or even no positive signal.
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Q4:Is Annexin V Kit able to detect cryopreserved cells directly?
It is not recommended to do so. Cryopreservation may lead to apoptosis and necrosis, resulting in inaccurate experimental results.
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Q5:Which Annexin V kit should be chosen for cell sample with green light?
If there is a DAPI channel in the flow cytometry, the E-CK-A258 Annexin V-APC/DAPI Apoptosis Detection Kit will be suitable. If not, the E-CK-A218 Annexin V-APC/7-AAD Apoptosis Detection Kit is avaliable.
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Q6:Can Annexin V kit be used on adherent cells?
Annexin V kit can be used on adherent cells, with the following caveat: 1. In late stage of apoptosis, cells may not able wo maintain adhesion, so floating cells should be collected for the test. 2. The dissociation of adherent cells should be handled gently to avoid cell damage caused by human operation. 3. Do not digest for too long with trypsin, avoid the usage of the trypsin contain EDTA. If EDTA-containing trypsin must be used, wash the cells to remove EDTA as much as possible.
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Q7:Is it able to detect blood samples with Annexin V assay kit?
It is not recommended to do so. Platelet and some other immune cells in blood may have exposed PS in normal state that will affect the test results.