Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application	Annexin V
Detection method	Fluorometric Method
Sample type	Cell samples
Assay time	40min
Detection instrument	Flow Cytometer
Dye Type	APC
Ex/Em (nm)	650/660
Channel set	APC
Other reagents required	PBS(E-BC-R187), Deionized water
Storage	2~8°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A151	Annexin V Binding Buffer (10×)
E-CK-A117	Annexin V-APC Reagent
E-CK-A218	Annexin V-APC/7-AAD Apoptosis Kit
E-CK-A258	Annexin V-APC/DAPI Apoptosis Kit
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)

Citations

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DOI: 10.1002/advs.202202914

PMID: 35981886
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DOI: 10.1038/s41467-022-30581-4

PMID: 35614066
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Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways

DOI: 10.1016/j.phrs.2022.106230
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DOI: 10.3389/fonc.2022.827891
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DOI: 10.3390/cancers14194809
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Analysis of the Roles of the ISLR2 Gene in Regulating the Toxicity of Zearalenone Exposure in Porcine Intestinal Epithelial Cells

DOI: 10.3390/toxins14090639

PMID: 36136577
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Calycosin-7-glucoside promotes mitochondria-mediated apoptosis in hepatocellular carcinoma by targeting thioredoxin 1 to regulate oxidative stress

DOI: 10.1016/j.cbi.2023.110411
Aging-US (2019) IF: 5.515
Circular RNA SMO sponges miR-338-3p to promote the growth of glioma by enhancing the expression of SMO

DOI: 10.18632/aging.102576
MOLECULAR NUTRITION & FOOD RESEARCH (2020) IF: 5.82
Lactobacillus Protects Against S. Typhimurium–Induced Intestinal Inflammation by Determining the Fate of Epithelial Proliferation and Differentiation

DOI: 10.1002/mnfr.201900655

Sample: Cell sample
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Knockdown of enhancer of rudimentary homolog inhibits proliferation and metastasis in ovarian cancer by regulating epithelial-mesenchymal transition

DOI: 10.1016/j.biopha.2020.109974

PMID: 32036222
Sample: Cell culture supernatant
Journal of Functional Biomaterials (2022) IF: 4.901
On-Demand Release of Fucoidan from a Multilayered Nanofiber Patch for the Killing of Oral Squamous Cancer Cells and Promotion of Epithelial Regeneration

DOI: 10.3390/jfb13040167

PMID: 36278636
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PARP inhibitors intervene DNA damage repair for the enhancement of tumor photodynamic therapy

DOI: 10.1016/j.pdpdt.2022.103058
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SOX4-activated lncRNA MCM3AP-AS1 aggravates osteoarthritis progression by modulating miR-149-5p/Notch1 signaling

DOI: 10.1016/j.cyto.2022.155805

PMID: 35202986
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2022) IF: 3.981
Curcumin-loaded zein/pectin nanoparticles: Caco-2 cellular uptake and the effects on cell cycle arrest and apoptosis of human hepatoma cells (HepG2)

DOI: 10.1016/j.jddst.2022.103497
ENDOCRINE JOURNAL (2021) IF: 2.349
Silencing long non-coding RNAs nicotinamide nucleotide transhydrogenase antisense RNA 1 inhibited papillary thyroid cancer cell proliferation, migration and invasion and promoted apoptosis via targeting miR-199a-5p

DOI: 10.1507/endocrj.EJ20-0353

PMID: 33612561
Sample: Cell（TPC-1 or IHH-4 cell）
International Journal of Medical Sciences (2020) IF: 2.523
Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury

DOI: 10.7150/ijms.45408

PMID: 33029099
Sample: Cell sample
JOURNAL OF MOLECULAR HISTOLOGY (2019) IF: 2.611
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DOI: 10.1007/s10735-019-09841-z
TECHNOLOGY IN CANCER RESEARCH & TREATMENT (2022) IF: 2.876
FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer:

DOI: 10.1177/15330338221118717
International Journal of Laboratory Hematology (2021) IF: 2.877
CD44 enhances adriamycin resistance in chronic myelogenous leukaemia cells K562

DOI: 10.1111/ijlh.13455

PMID: 33411349
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE (2022) IF: 1.848
Trifluoperazine Synergistically Potentiates Bortezomib-Induced Anti-Cancer Effect in Multiple Myeloma via Inhibiting P38 MAPK/NUPR1

DOI: 10.1620/tjem.2022.J044

PMID: 35644544

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