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Annexin V-FITC/PI Apoptosis Kit

  • Cat.No.:E-CK-A211

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Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application Annexin V
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer
Dye Type FITC
Ex/Em (nm) 490/530
Channel set FITC
Other reagents required PBS(E-BC-R187), Deionized water
Storage 2~8°C
Shipping Ice bag
Expiration date 12 months

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Aging (Albany NY) (2020) IF: 4.831
    Demethoxycurcumin analogue DMC-BH inhibits orthotopic growth of glioma stem cells by targeting JNK/ERK signaling

    DOI: 10.18632/aging.103531

    PMID: 32710727

    Sample: Cell sample
  2. Microorganisms (2020) IF: 4.152
    Human Antimicrobial Peptide Hepcidin 25-Induced Apoptosis in Candida albicans

    DOI: 10.3390/microorganisms8040585

    PMID: 32316661

    Sample: Cell sample
  3. Cancer Chemotherapy and Pharmacology (2020) IF: 3.008
    Anticancer e ects of brusatol in nasopharyngeal carcinoma through suppression of the Akt/mTOR signaling pathway

    DOI: 10.1007/s00280-020-04083-3

    Sample: Cell lysate
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Verified Customer

w**tSubmitted [ Dec 04 2019 ]

  • Application:FCM
  • Description:i like this produce, because the separation of apoptotic cells from normal cells is good. I get results very well
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