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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Biotin Anti-Mouse CD279/PD-1 Antibody[29F.1A12]

Biotin Anti-Mouse CD279/PD-1 Antibody[29F.1A12]

Uniprot : Q02242

  • Cat.No.:E-AB-F1131B

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1131B

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  • 25μg
  • 100μg
Price: $55
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Antigen Details

Alternate Names PD-1, Programmed Death-1
Swissprot Q02242
References
1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2009.

Product Details

Form Liquid
Clone No. 29F.1A12(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control Biotin Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833B]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Biotin

How to select the appropriate detection channel through the spectrogram?

Background

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More

Citations

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