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cAMP(Cyclic adenosine monophosphate) ELISA Kit

  • Cat.No.:E-EL-0056

  • Reactivity: Universal

To Purchase E-EL-0056

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes cAMP in samples. No significant cross-reactivity or interference between cAMP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level cAMP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level cAMP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 5.40 14.10 37.40 5.90 15.00 34.90
Standard deviation 0.30 0.70 2.00 0.40 0.90 1.80
CV (%) 5.56 4.96 5.35 6.78 6.00 5.16

Recovery

The recovery of cAMP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 95
EDTA plasma(n=8) 86-100 92
Cell culture media(n=8) 87-100 92

Linearity

Samples were spiked with high concentrations of cAMP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCyclic adenosine monophosphate, cAMP, cyclic AMP, 3'-5'-cyclic adenosine monophosphate

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. ACS chemical neuroscience (2018) IF: 4.211
    The Inhibition by Guanfu Base A of Neuropathic Pain Mediated by P2Y12 Receptor in Dorsal Root Ganglia

    DOI: 10.1021/acschemneuro.8b00399

    PMID: 30475578

    Sample: Cell culture supernatant
  2. International journal of molecular sciences (2018) IF: 3.687
    Developmental Exposure to Atrazine Impairs Spatial Memory and Downregulates the Hippocampal D1 Dopamine Receptor and cAMP-Dependent Signaling Pathway in Rats

    DOI: 10.3390/ijms19082241

    PMID: 30065202

    Sample: Tissue homogenate
  3. International journal of oncology (2019) IF: 3.333
    CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells

    DOI: 10.3892/ijo.2018.4651

    PMID: 30535454

    Sample: Cell lysate
  4. Acta Pharmacologica Sinica (2019) IF: 3.562
    Osthole prevents tamoxifen-induced liver injury in mice

    DOI: 10.1038/s41401-018-0171-y

    PMID: 30315252

    Sample: Tissue homogenate
  5. Front. Aging Neurosci. (2019) IF: 3.633
    Royal Jelly Alleviates Cognitive Deficits and b-Amyloid Accumulation in APP/PS1 Mouse Model Via Activation of the cAMP/PKA/CREB/BDNF Pathway and Inhibition of Neuronal Apoptosis

    DOI: 10.3389/fnagi.2018.00428

    PMID: 30687079

    Sample: Tissue homogenate
  6. Fitoterapia (2020) IF: 2.527
    Anti-melanogenic effects of epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG) and gallocatechin-3-gallate (GCG) via down-regulation of cAMP/CREB /MITF signaling pathway in B16F10 melanoma cells

    DOI: 10.1016/j.fitote.2020.104634

    PMID: 32454171

    Sample: Cell culture supernatant
  7. Journal of cellular biochemistry (2019) IF: 2.959
    A novel role and mechanism of cystic fibrosis transmembrane conductance regulator in bisphenol A-Induced prostate cancer

    DOI: 10.1002/jcb.28156

    PMID: 30485539

    Sample: Cell culture supernatant
  8. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system

    DOI: 10.3892/etm.2020.8759

    PMID: 32742325

    Sample: Cell lysate
  9. International journal of stem cells (2020) IF: 1.94
    Estrogen Promotes cAMP Production in Mesenchymal Stem Cells by Regulating ADCY2

    DOI: 10.15283/ijsc19139

    PMID: 32114743

    Sample: Cell culture supernatant
  10. BMC medical genetics (2018) IF: 1.913
    Identification of a novel GNAS mutation in a case of pseudohypoparathyroidism type 1A with normocalcemia

    DOI: 10.1186/s12881-018-0648-z

    PMID: 30060753

    Sample: Cell culture supernatant
  11. Experimental and therapeutic medicine (2018) IF: 1.41
    Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin-angiotensin system activity

    DOI: 10.3892/etm.2018.6734

    PMID: 30402152

    Sample: Plasma,Tissue homogenate
  12. BMC medical genetics (2018) IF: 1.913
    Identification of a novel GNAS mutation in a case of pseudohypoparathyroidism type 1A with normocalcemia.

    DOI: 10.1186/s12881-018-0648-z

    PMID: 30060753

    Sample: Cell
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