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DA(Dopamine) ELISA Kit

  • Cat.No.:E-EL-0046

  • Reactivity: Universal

To Purchase E-EL-0046

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes DA in samples. No significant cross-reactivity or interference between DA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of DA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DA. During the reaction, DA in the sample or standard competes with a fixed amount of DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level DA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level DA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 93.20 168.70 786.20 94.20 159.90 725.00
Standard deviation 6.10 7.40 29.10 5.30 9.00 37.00
CV (%) 6.55 4.39 3.70 5.63 5.63 5.10

Recovery

The recovery of DA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-110 101
EDTA plasma(n=8) 97-111 102
Cell culture media(n=8) 92-103 98

Linearity

Samples were spiked with high concentrations of DA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms2-(3, 4-dihydroxyphenyl)ethylamine, 3, 4-dihydroxyphenethylamine, 3-hydroxytyramine, DA, Intropin, Revivan, Oxytyramine

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cells (2020) IF: 4.366
    Evaluation of Priming Efficiency of Forskolin in Tissue-Specific Human Mesenchymal Stem Cells into Dopaminergic Neurons: An In Vitro Comparative Study

    DOI: 10.3390/cells9092058

    PMID: 32917012

    Sample: Cell culture supernatant
  2. Chemosphere (2019) IF: 4.427
    Study on neurotoxicity of dinotefuran, thiamethoxam and imidacloprid against Chinese lizards (Eremias argus)

    DOI: 10.1016/j.chemosphere.2018.11.016

    PMID: 30419375

    Sample: Plasma
  3. Scientific Reports (2020) IF: 3.998
    The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals

    DOI: 10.1038/s41598-020-72098-0

    PMID: 32929110

    Sample: Tissue homogenate
  4. Frontiers in physiology (2019) IF: 3.367
    Oleanolic Acid Mitigates 6-Hydroxydopamine Neurotoxicity by Attenuating Intracellular ROS in PC12 Cells and Striatal Microglial Activation in Rat Brains

    DOI: 10.3389/fphys.2019.01059

    PMID: 31496954

    Sample: Tissue homogenate
  5. International journal of molecular sciences (2018) IF: 3.687
    Developmental Exposure to Atrazine Impairs Spatial Memory and Downregulates the Hippocampal D1 Dopamine Receptor and cAMP-Dependent Signaling Pathway in Rats

    DOI: 10.3390/ijms19082241

    PMID: 30065202

    Sample: Tissue homogenate
  6. International Journal of Molecular Sciences (2017) IF: 3.226
    Dopaminergic Dysfunction In Mammalian Dopamine Neurons Induced By Simazine Neurotoxicity.

    DOI: 10.3390/ijms18112404

    PMID: 29137186

    Sample: Cell culture supernatant
  7. American Journal of Hypertension (2016) IF: 3.541
    A Novel Swine Model Of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well To Renal Denervation.

    DOI: 10.1093/ajh/hpv066

    PMID: 25958303

    Sample: Plasma,Urine
  8. Metabolic Brain Disease (2019) IF: 2.726
    Effects of bromelain on motor responses following intra-medial forebrain bundle 6-OHDA injection in rat model of parkinsonism

    DOI: 10.1007/s11011-019-00462-9

    PMID: 31332728

    Sample: Tissue homogenate
  9. Environmental toxicology (2019) IF: 2.491
    Oxidative stress and neuromodulatory effects of deltamethrin and its combination with insect repellents in rats

    DOI: 10.1002/tox.22741

    PMID: 30884102

    Sample: Serum
  10. Neuroscience Bulletin (2016) IF: 2.624
    miR-137 And miR-491 Negatively Regulate Dopamine Transporter Expression And Function In Neural Cells.

    DOI: 10.1007/s12264-016-0061-6

    PMID: 27628529

    Sample: Cell lysate
  11. Journal of Ethnopharmacology (2016) IF: 2.981
    Venenum Bufonis Induces Rat Neuroinflammation By Activiating NF-κB Pathway And Attenuation Of BDNF.

    DOI: 10.1016/j.jep.2016.03.049

    PMID: 27013094

    Sample: Tissue homogenate
  12. Indian Journal of Forensic Medicine & Toxicology (2019)
    The Association between Dopamine and Oxidative Stress for Methamphetamine Iraqi Addicts

    DOI: 10.5958/0973-9130.2019.00364.5

    PMID: 99999999

    Sample: Serum
  13. Turkish Journal of Science (2019)
    The Effect of Peripheral Adropin Application on Hypothalamic Pituitary Adrenal Axis in Rats

    DOI: 99999999

    PMID: 99999999

    Sample: Tissue homogenate
  14. Journal of Kerbala University (2019)
    EFFECT OF TOPIRAMATE DRUG ON NEUROTRANSMITTERS LEVELS (ACETYLCHOLINE AND DOPAMINE) IN PREGNANT ALBINO RATS

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  15. Research Journal of Pharmacy and Technology (2017)
    Symbiotic effect of sodium selenite and vitamin E against lithium carbonate induced brain toxicity in male rats

    DOI: 10.5958/0974-360X.2017.00293.1

    PMID: 99999999

    Sample: Serum
  16. Journal of Pharmaceutical Sciences and Research (2019)
    Effect of chronic toxoplasmosis on levels of some neurotransmitters (Dopamine, Adrenaline, and Noradrenaline) in human Serum

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
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