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Catalog number:E-TO-E003
Sensitivity: 3.00 ppb
Sample type: Grain,Feed
Lead Time: 7~10 daysWelcome to order from local distributors.
Add to cart Compare Bulk request ManualTest principle
This kit uses Competitive-ELISA as the method. It can detect Deoxynivalenol (DON) in samples, such as grain and feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the reaction, DON in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-DON antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of DON. The concentration of DON in the samples can be calculated by comparing the OD of the samples to the standard curve.
Technical indicator
Sensitivity: 3 ppb (ng/mL)
Reaction mode: 37℃, 30 min~30 min~15 min
Detection limit: Grain,Feed---150 ppb
Cross-reactivity: Deoxynivalenol (C15H20O6)---100%; 3-Acetyldeoxynivalenol (C17H22O6)---<1%,
Sample recovery rate: 85%±15%
Kits components
Item |
Specifications |
ELISA Microtiter plate |
96 wells |
Standard Liquid |
1 mL each (0 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb, 243 ppb) |
HRP Conjugate |
11 mL |
Antibody Working Solution |
5.5 mL |
Substrate Reagent A |
6 mL |
Substrate Reagent B |
6 mL |
Stop Solution |
6 mL |
20×Concentrated Wash Buffer |
40 mL |
2×Reconstitution Buffer |
50 mL |
Plate Sealer |
3 pieces |
Sealed Bag |
1 piece |
Manual |
1 copy |
Other supplies required
Instrument: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).
High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).
Storage and valid period
Store at 2~8℃ for 1 year. Avoid freeze.
Please store the opened kit at 2~8℃, protect from light and moisture. The valid period is 1 months.
Expiry date: expiration date is on the packing box.