Test principle
This kit uses Competitive-ELISA as the method. It
can detect Deoxynivalenol (DON) in samples, such as grain and feed,
etc. This kit is composed of ELISA Microtiter plate, HRP
conjugate, antibody working solution, standard
and other supplementary reagents. The microtiter plate provided in this kit has
been pre-coated with coupled antigen. During the reaction, DON in the samples
or standard competes with coupled antigen on the solid phase supporter for
sites of anti-DON antibody. Then Horseradish Peroxidase (HRP) conjugate is
added to each microtiter plate well, and TMB substrate is added for color
development. There is a negative correlation between the OD value of samples
and the concentration of DON. The concentration of DON in the samples can be
calculated by comparing the OD of the samples to the standard curve.
Technical indicator
Sensitivity: 3 ppb (ng/mL)
Reaction mode: 37℃, 30 min~30 min~15
min
Detection limit: Grain,Feed---150
ppb
Cross-reactivity: Deoxynivalenol
(C15H20O6)---100%;
3-Acetyldeoxynivalenol (C17H22O6)---＜1%,
Sample recovery rate: 85%±15%
Kits components
|
Item
|
Specifications
|
|
ELISA Microtiter plate
|
96 wells
|
|
Standard Liquid
|
1 mL each
(0 ppb, 3 ppb, 9
ppb, 27 ppb, 81 ppb, 243 ppb)
|
|
HRP Conjugate
|
11 mL
|
|
Antibody Working Solution
|
5.5 mL
|
|
Substrate Reagent A
|
6 mL
|
|
Substrate Reagent B
|
6 mL
|
|
Stop Solution
|
6 mL
|
|
20×Concentrated
Wash Buffer
|
40 mL
|
|
2×Reconstitution Buffer
|
50 mL
|
|
Plate
Sealer
|
3
pieces
|
|
Sealed
Bag
|
1
piece
|
|
Manual
|
1
copy
|
Other supplies required
Instrument: Microplate reader, Printer,
Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).
High-precision
transferpettor: Single channel (20-200 μL, 100-1000
μL), Multichannel (300 μL).
Storage
and valid period
Store at
2~8℃ for 1 year. Avoid freeze.
Please store the opened kit at 2~8℃, protect from light and moisture.
The valid period is 1 months.
Expiry date: expiration date is on the packing box.
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