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Current Position:Home>>Cell Products>>Cell Proliferation/Cytotoxicity/Viability>> Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Citations(44)

  • Cat.No.:E-CK-A362

Applications:Viability/Proliferation/Cytotoxicity

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Detection principle

WST-8 is a compound similar to MTT, which can be reduced to orange formazan by some dehydrogenase in mitochondria in the presence of electron coupling reagent. The amount of formazan produced is directly proportional to the number of living cells. By measuring the absorbance at 450 nm, the amount of living cells can be calculated indirectly.

General Information

Application Viability/Proliferation/Cytotoxicity
Detection method Colorimetric Method
Sample type Cell samples
Detection instrument Microplate reader
Storage 2~8°C or -20°C , shading light
Shipping Ice bag
Expiration date 24 months

Citations

  1. Molecular Cancer (2022) IF: 41.444
    CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathway

    DOI: 10.1186/s12943-022-01603-y

    PMID: 35681235
  2. Cellular & Molecular Immunology (2023) IF: 22.096
    HTR2A agonists play a therapeutic role by restricting ILC2 activation in papain-induced lung inflammation

    DOI: 10.1038/s41423-023-00982-6

    PMID: 36823235
  3. JOURNAL OF HAZARDOUS MATERIALS (2022) IF: 14.224
    Polystyrene nanoparticle exposure supports ROS-NLRP3 axis-dependent DNA-NET to promote liver inflammation

    DOI: 10.1016/j.jhazmat.2022.129502

    PMID: 35868089
  4. JOURNAL OF PINEAL RESEARCH (2022) IF: 12.081
    Melatonin alleviates the heat stress-induced impairment of Sertoli cells by reprogramming glucose metabolism

    DOI: 10.1111/jpi.12819

    PMID: 35906194
  5. Frontiers in Immunology (2023) IF: 8.786
    Identification and validation of a prognostic signature of autophagy, apoptosis and pyroptosis-related genes for head and neck squamous cell carcinoma: to imply therapeutic choices of HPV negative patients

    DOI: 10.3389/fimmu.2022.1100417
  6. Frontiers in Immunology (2022) IF: 8.786
    AQP2 Promotes Astrocyte Activation by Modulating the TLR4/NFκB-p65 Pathway Following Intracerebral Hemorrhage.

    DOI: 10.3389/fimmu.2022.847360
  7. Materials Today Bio (2021) IF: 7.348
    Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer

    DOI: 10.1016/j.mtbio.2021.100189
  8. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Efficacy of WWQ-131, a highly selective JAK2 inhibitor, in mouse models of myeloproliferative neoplasms

    DOI: 10.1016/j.biopha.2022.113884

    PMID: 36306591
  9. Materials Today Bio (2022) IF: 10.761
    Controllable graphene oxide-based biocompatible hybrid interface as an anti-fibrotic coating for metallic implants

    DOI: 10.1016/j.mtbio.2022.100326

    PMID: 35761844
  10. LWT-FOOD SCIENCE AND TECHNOLOGY (2022) IF: 6.056
    Extraction, identification and anti-photoaging activity evaluation of collagen peptides from silver carp (Hypophthalmichthys molitrix) skin

    DOI: 10.1016/j.lwt.2022.114384
  11. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Regulating Th17/Treg Balance Contributes to the Therapeutic Effect of Ziyuglycoside I on Collagen-Induced Arthritis

    DOI: 10.3390/ijms232416105
  12. PHYTOMEDICINE (2022) IF: 6.656
    Luteolin ameliorates hypoxia-induced pulmonary hypertension via regulating HIF-2α-Arg-NO axis and PI3K-AKT-eNOS-NO signaling pathway

    DOI: 10.1016/j.phymed.2022.154329

    PMID: 35843187
  13. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2022) IF: 5.085
    Adipose improves muscular atrophy caused by Sirtuin1 deficiency by promoting mitochondria synthesis

    DOI: 10.1016/j.biocel.2022.106246

    PMID: 35738524
  14. ACS Chemical Biology (2022) IF: 5.1
    YTHDF3 Facilitates eIF2AK2 and eIF3A Recruitment on mRNAs to Regulate Translational Processes in Oxaliplatin-Resistant Colorectal Cancer

    DOI: 10.1021/acschembio.2c00131

    PMID: 35708211
  15. BIOORGANIC CHEMISTRY (2022) IF: 5.307
    Cytotoxic steroidal saponins from the rhizomes of Paris fargesii var. petiolata

    DOI: 10.1016/j.bioorg.2022.106305
  16. PHYTOMEDICINE (2022) IF: 5.34
    Protective role of casuarinin from Melastoma malabathricum against a mouse model of 5-fluorouracil–induced intestinal mucositis: Impact on inflammation and gut microbiota dysbiosis

    DOI: 10.1016/j.phymed.2022.154092
  17. CERAMICS INTERNATIONAL (2022) IF: 5.532
    Composite monetite/amorphous calcium phosphate bone cement promotes bone regeneration

    DOI: 10.1016/j.ceramint.2022.10.296
  18. Frontiers in Pharmacology (2021) IF: 5.811
    Shaoyao-Gancao Decoction Ameliorates the Inflammation State in Polycystic Ovary Syndrome Rats via Remodeling Gut Microbiota and Suppressing the TLR4/NF-κB Pathway

    DOI: 10.3389/fphar.2021.670054

    PMID: 34054541

    		 Sample: Cell culture supernatant
  19. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 5.875
    Safflower oil body nanoparticles deliver hFGF10 to hair follicles and reduce microinflammation to accelerate hair regeneration in androgenetic alopecia

    DOI: 10.1016/j.ijpharm.2022.121537

    PMID: 35150848
  20. PHYTOCHEMISTRY (2022) IF: 4.004
    Cytotoxic steroidal glycosides from the rhizomes of Paris polyphylla var. yunnanensis

    DOI: 10.1016/j.phytochem.2022.113577
  21. ONCOLOGY REPORTS (2023) IF: 4.136
    The PTBP1‑NCOA4 axis promotes ferroptosis in liver cancer cells

    DOI: 10.3892/or.2023.8482
  22. Human Cell (2022) IF: 4.174
    The α5-nAChR/PD-L1 axis facilitates lung adenocarcinoma cell migration and invasion

    DOI: 10.1007/s13577-022-00709-1

    PMID: 35593989
  23. MOLECULES (2021) IF: 4.412
    New Steroidal Saponins Isolated from the Rhizomes of Paris mairei

    DOI: 10.3390/molecules26216366

    PMID: 34770773
  24. MOLECULES (2022) IF: 4.412
    Mechanism of Action of Flavonoids of Oxytropis falcata on the Alleviation of Myocardial Ischemia–Reperfusion Injury

    DOI: 10.3390/molecules27051706
  25. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    Discoidin Domain Receptors 1 Inhibition Alleviates Osteoarthritis via Enhancing Autophagy

    DOI: 10.3390/ijms21196991

    PMID: 32977456

    		 Sample: Cell sample
  26. BMC CANCER (2022) IF: 4.638
    LAP3 contributes to IFN-γ-induced arginine depletion and malignant transformation of bovine mammary epithelial cells

    DOI: 10.1186/s12885-022-09963-w

    PMID: 35941558
  27. MOLECULES (2022) IF: 4.927
    Ajuforrestin A, an Abietane Diterpenoid from Ajuga ovalifolia var. calanthe, Induces A549 Cell Apoptosis by Targeting SHP2

    DOI: 10.3390/molecules27175469

    PMID: 36080236
  28. Scientific Reports (2023) IF: 4.996
    Long non-coding RNA KCNQ1OT1 alleviates postmenopausal osteoporosis by modulating miR-421-3p/mTOR axis

    DOI: 10.1038/s41598-023-29546-4
  29. Eye and Vision (2022) IF: 3.257
    The ghrelin-GHSR-1a pathway inhibits high glucose-induced retinal angiogenesis in vitro by alleviating endoplasmic reticulum stress

    DOI: 10.1186/s40662-022-00291-5

    PMID: 35668539
  30. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322
    IGF2BP3-NRF2 axis regulates ferroptosis in hepatocellular carcinoma

    DOI: 10.1016/j.bbrc.2022.08.040

    PMID: 36030651
  31. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
    Guizhi-Shaoyao-Zhimu decoction attenuates bone erosion in rats that have collagen-induced arthritis via modulating NF‐κB signalling to suppress osteoclastogenesis

    DOI: 10.1080/13880209.2021.1876100
  32. Cancer Management and Research (2022) IF: 3.602
    CSTF2 Acts as a Prognostic Marker Correlated with Immune Infiltration in Hepatocellular Carcinoma

    DOI: 10.2147/CMAR.S359545

    PMID: 36117731
  33. PLoS One (2023) IF: 3.752
    Inhibition of mycobacteria proliferation in macrophages by low cisplatin concentration through phosphorylated p53-related apoptosis pathway

    DOI: 10.1371/journal.pone.0281170
  34. JOURNAL OF PHARMACY AND PHARMACOLOGY (2022) IF: 3.765
    Lianhuaqingwen alleviates p53-mediated apoptosis in alveolar epithelial cells to prevent LPS-induced ALI

    DOI: 10.1093/jpp/rgac035

    PMID: 35640566
  35. Current Medical Science (2022) IF: 2.135
    Downregulation of Astrocyte Elevated Gene-1 Expression Combined with All-Trans Retinoic Acid Inhibits Development of Vasculogenic Mimicry and Angiogenesis in Glioma

    DOI: 10.1007/s11596-022-2517-4

    PMID: 35201552
  36. JOURNAL OF CLINICAL LABORATORY ANALYSIS (2022) IF: 2.352
    Arctigenin inhibits apoptosis, extracellular matrix degradation, and inflammation in human nucleus pulposus cells by up-regulating miR-483-3p

    DOI: 10.1002/jcla.24508

    PMID: 35689566
  37. Experimental and Therapeutic Medicine (2022) IF: 2.447
    Verbascoside potentiates the effect of tyrosine kinase inhibitors on the induction of apoptosis and oxidative stress via the Abl-mediated MAPK signalling pathway in chronic myeloid leukaemia

    DOI: 10.3892/etm.2022.11441

    PMID: 35837042
  38. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism

    DOI: 10.3892/etm.2021.10407
  39. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2022) IF: 2.464
    Anti-hepatic Fibrosis Potential of Ailanthone, A Quassinoid Isolated from Ailanthus altissima

    DOI: 10.1007/s43450-022-00340-z
  40. HEREDITAS (2022) IF: 2.595
    Arecoline promotes proliferation and migration of human HepG2 cells through activation of the PI3K/AKT/mTOR pathway

    DOI: 10.1186/s41065-022-00241-0

    PMID: 35836300
  41. MEDICAL SCIENCE MONITOR (2022) IF: 2.649
    Expression of Long Non-Coding RNA (lncRNA) SNHG5 in Patients with Refractory Diabetic Macular Edema and Its Regulatory Mechanism

    DOI: 10.12659/MSM.932996
  42. Computational and Mathematical Methods in Medicine (2022) IF: 2.809
    Apatinib Functioned as Tumor Suppressor of Synovial Sarcoma through Regulating miR-34a-5p/HOXA13 Axis

    DOI: 10.1155/2022/7214904

    PMID: 36276991
  43. Molecular Medicine Reports (2021) IF: 2.952
    FPR2 serves a role in recurrent spontaneous abortion by regulating trophoblast function via the PI3K/AKT signaling pathway

    DOI: 10.3892/mmr.2021.12478

    PMID: 34608500
  44. ACTA PARASITOLOGICA (2021) IF: 1.44
    Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model

    DOI: 10.1007/s11686-021-00434-z

    PMID: 34143400
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