Click here to view cell apoptosis assay products
×

Elab Fluor® Violet 450 Anti-Rat CD45 Antibody[OX-1]

Uniprot : P04157
  • Cat.No.:E-AB-F1227Q

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1227Q

Size:
  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $60
Qty:

Antigen Details

Alternate Names Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen, LCA, T200, Ly-5,Ptprc
Swissprot P04157
Gene ID 19265
References
1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.

Product Details

Form Liquid
Clone No. OX-1(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control Elab Fluor® Violet 450 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792Q]
Reactivity Rat
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Elab Fluor® Violet 450
Elab Fluor® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for 10 Test Free Trial FCM Antibody
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: