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Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)

Citations(3)Uniprot : tjj
  • Cat.No.:E-BC-K234-M

  • Detection instrument: Microplate reader(500-510 nm)

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Detection principle

Glucose oxidase can catalyze the oxidation of glucose to gluconic acid to produce hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide and oxidizes pigment sources to form colored substances. Measure the OD value at 505 nm and glucose content can be calculated indirectly.

Performance characteristics

Synonyms Glu
Sample type Serum,plasma
Sensitivity 0.04 mmol/L
Detection range 0.04-30 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 2.3%Average intra-assay CV: 1.9%
Other instruments required Vortex mixer, Micropipettor, Incubator
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 6 months

Dilution of sample


It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.04-30 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

Rat serum

1

Human plasma

1

Note: The diluent is normal saline (0.9% NaCl) .


Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Theriogenology (2020) IF: 2.094
    Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model

    DOI: 10.1016/j.theriogenology.2020.09.023

    PMID: 32980686

    Sample: Serum
  2. Cancer Management and Research (2020) IF: 2.886
    Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis

    DOI: 10.2147/CMAR.S242135

    PMID: 32669871

    Sample: Cell culture supernatant
  3. Environmental Science and Pollution Research (2019) IF: 2.8
    Isolation, characterization, and therapeutic activity of bergenin from marlberry (Ardisia colorata Roxb.) leaf on diabetic testicular complications in Wistar albino rats

    DOI: 10.1007/s11356-019-04139-9

    PMID: 30648235

    Sample: Serum
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