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Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)

Cat.No.:E-BC-K234-M
Detection instrument: Microplate reader(500-510 nm)

Detection method: Colorimetric method

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Detection principle

Glucose oxidase can catalyze the oxidation of glucose to gluconic acid to produce hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide and oxidizes pigment sources to form colored substances. Measure the OD value at 505 nm and glucose content can be calculated indirectly.

Performance characteristics

Synonyms	Glu
Sample type	Serum,plasma,whole blood
Sensitivity	0.04 mmol/L
Detection range	0.04-30 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 2.300%Average intra-assay CV: 1.900%
Other instruments required	Vortex mixer, Micropipettor, Incubator
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.04-30 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Rat serum	1
Human plasma	1

Note: The diluent is normal saline (0.9% NaCl) .

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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DOI: 10.3389/fendo.2021.668193
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Canagliflozin Modulates Hypoxia-Induced Metastasis, Angiogenesis and Glycolysis by Decreasing HIF-1α Protein Synthesis via AKT/mTOR Pathway

DOI: 10.3390/ijms222413336
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Microorganisms (2021) IF: 4.128

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DOI: 10.1530/REP-20-0381
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PMID: 34915104	Sample: serum

THERIOGENOLOGY (2020) IF: 2.094

Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model

DOI: 10.1016/j.theriogenology.2020.09.023
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Reviews/Q&A

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Reviews
Q&A

M**********dSubmitted [ Aug 12 2022 ]

Asked: Is it possible to measure the glucose concentration in juice?

adminSubmitted [ Aug 12 2022 ]

Answered: Yes, it is. There is no species distinction for samples for metabolism assays. But it's better to make a sample control for your samples especially for the first time.

D********dSubmitted [ Aug 01 2022 ]

Asked: How long can I keep the working solution?

adminSubmitted [ Aug 01 2022 ]

Answered: I't better to finishing using the working solution as soon as possible. If it's not available to use it immediately, the working solution must be protect from light at 2-8 degree, and no more than 24 hours.

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