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Cat.No.:E-EL-0071
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes GnRH in samples. No significant cross-reactivity or interference between GnRH and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GnRH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GnRH were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 281.20 | 937.80 | 2978.30 | 281.90 | 856.30 | 3080.80 |
Standard deviation | 14.60 | 48.80 | 131.00 | 18.00 | 41.10 | 114.00 |
CV (%) | 5.19 | 5.20 | 4.40 | 6.39 | 4.80 | 3.70 |
The recovery of GnRH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 93-111 | 101 |
EDTA plasma(n=8) | 88-100 | 95 |
Cell culture media(n=8) | 87-97 | 92 |
Samples were spiked with high concentrations of GnRH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | GNRH1, GRH, HH12, LHRH, LNRH, Luliberin |
Research Area | Developmental biology, Signal transduction |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
Sample: GT1-7 cells |
PMID: 34030373 |
Sample: Serum |
PMID: 33396074 |
Sample: Serum |
PMID: 32388187 |
Sample: Cell culture supernatant |
DOI: 10.2147/IJN.S348673 |
|
PMID: 35401001 |
Sample: serum |
PMID: 35658231 |
Sample: serum |
PMID: 35626727 |
Sample: serum |
Sample: Tissue homogenate |
PMID: 35341824 |
Sample: serum |
PMID: 33577849 |
Sample: Tissue homogenate |
PMID: 31519099 |
Sample: Serum |
PMID: 30833257 |
Sample: Plasma |
PMID: 31874432 |
Sample: Plasma |
PMID: 34280466 |
Sample: Serum |
PMID: 34411681 |
Sample: serum |
Sample: plasma |
PMID: 34656705 |
Sample: plasma |
Sample: serum |
PMID: 31689472 |
Sample: Serum |
PMID: 31654709 |
Sample: Serum |
Sample: plasma |
Sample: serum |
Sample: Plasma |
A***********hSubmitted [ Jun 13 2023 ]
Asked: I would like a quote for this item, if you have a bovine GnRH kit, it is most appropriate for our assay GnRH(Gonadotropin Releasing Hormone) ELISA Kit Cat.No.:E-EL-0071 Also I would like to ask for a quote for GnRH-HRP conjugate detection reagent separately, Could you provide that separately please?
Reply
adminSubmitted [ Jun 13 2023 ]
Answered: Hello Ahmed, I will reply you via email later.