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GnRH(Gonadotropin Releasing Hormone) ELISA Kit

  • Cat.No.:E-EL-0071

  • Reactivity: Universal

To Purchase E-EL-0071

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes GnRH in samples. No significant cross-reactivity or interference between GnRH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GnRH. During the reaction, GnRH in the sample or standard competes with a fixed amount of GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GnRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GnRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GnRH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GnRH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 281.20 937.80 2978.30 281.90 856.30 3080.80
Standard deviation 14.60 48.80 131.00 18.00 41.10 114.00
CV (%) 5.19 5.20 4.40 6.39 4.80 3.70


The recovery of GnRH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-111 101
EDTA plasma(n=8) 88-100 95
Cell culture media(n=8) 87-97 92


Samples were spiked with high concentrations of GnRH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsGNRH1, GRH, HH12, LHRH, LNRH, Luliberin
Research AreaDevelopmental biology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

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  1. Environmental Pollution (2020) IF: 6.792
    Enrichment of imidacloprid and its metabolites in lizards and its toxic effects on gonads

    DOI: 10.1016/j.envpol.2019.113748

    PMID: 31874432

    Sample: Plasma
  2. Chemosphere (2019) IF: 5.108
    T-2 toxin impairs male fertility by disrupting hypothalamic-pituitary-testis axis and declining testicular function in mice

    DOI: 10.1016/j.chemosphere.2019.06.145

    PMID: 31519099

    Sample: Serum
  3. Toxicology (2021) IF: 4.221
    Environmentally relevant perinatal exposure to DBP disturbs testicular development and puberty onset in male mice

    DOI: 10.1016/j.tox.2021.152860

    PMID: 34280466

    Sample: Serum
  4. Food and Chemical Toxicology (2020) IF: 4.679
    Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats

    DOI: 10.1016/j.fct.2019.110897

    PMID: 31654709

    Sample: Serum
  5. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    In utero exposure to DBP stimulates release of GnRH by increasing the secretion of PGE2 in the astrocytes of the hypothalamus in the offspring mice

    DOI: 10.1016/j.ecoenv.2020.110698

    PMID: 32388187

    Sample: Cell culture supernatant
  6. Science of The Total Environment (2019) IF: 4.61
    Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus

    DOI: 10.1016/j.scitotenv.2019.02.419

    PMID: 30833257

    Sample: Plasma
  7. Toxicology Letters (2019) IF: 3.499
    Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats

    DOI: 10.1016/j.toxlet.2019.10.024

    PMID: 31689472

    Sample: Serum
  8. Autonomic Neuroscience-Basic & Clinical (2015) IF: 2.225
    Brain Thromboxane A2 Via Arachidonic Acid Cascade Induces The Hypothalamic-Pituitary-Gonadal Axis Activation In Rats.

    DOI: 10.1016/j.autneu.2015.02.005

    PMID: 25784152

    Sample: Plasma
  9. Ecotoxicology and Environmental Safety (2021) IF: 4.872
    Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels Unio tigridis and snails Viviparous benglensis.

    DOI: 10.1016/j.ecoenv.2020.111748

    PMID: 33396074

    Sample: Serum
  10. Ecotoxicology and Environmental Safety (2021) IF: 4.872
    Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice

    DOI: 10.1016/j.ecoenv.2020.111748

    PMID: 33396074

    Sample: Serum
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