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GnRH(Gonadotropin Releasing Hormone) ELISA Kit

  • Cat.No.:E-EL-0071

  • Reactivity: Universal

To Purchase E-EL-0071

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes GnRH in samples. No significant cross-reactivity or interference between GnRH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GnRH. During the reaction, GnRH in the sample or standard competes with a fixed amount of GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GnRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GnRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GnRH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GnRH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 281.20 937.80 2978.30 281.90 856.30 3080.80
Standard deviation 14.60 48.80 131.00 18.00 41.10 114.00
CV (%) 5.19 5.20 4.40 6.39 4.80 3.70

Recovery

The recovery of GnRH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-111 101
EDTA plasma(n=8) 88-100 95
Cell culture media(n=8) 87-97 92

Linearity

Samples were spiked with high concentrations of GnRH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsGNRH1, GRH, HH12, LHRH, LNRH, Luliberin
Research AreaDevelopmental biology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Molecular Therapy-Nucleic Acids (2021) IF: 8.8859
    The epigenetic role of HTR1A antagonist in facilitating GnRH expression for pubertal initiation control

    DOI: 10.1016/j.omtn.2021.05.014

    Sample: GT1-7 cells
  2. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Effects of acute exposure to microcystins on hypothalamic-pituitary-adrenal (HPA), -gonad (HPG) and -thyroid (HPT) axes of female rats

    DOI: 10.1016/j.scitotenv.2021.145196

    PMID: 34030373

    Sample: Serum
  3. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291
    Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice

    DOI: 10.1016/j.ecoenv.2020.111748

    PMID: 33396074

    Sample: Serum
  4. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291
    In utero exposure to DBP stimulates release of GnRH by increasing the secretion of PGE2 in the astrocytes of the hypothalamus in the offspring mice

    DOI: 10.1016/j.ecoenv.2020.110698

    PMID: 32388187

    Sample: Cell culture supernatant
  5. International Journal of Nanomedicine (2022) IF: 6.4
    Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway

    DOI: 10.2147/IJN.S348673

    PMID: 35401001

    Sample: serum
  6. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway

    DOI: 10.1016/j.biopha.2022.113061

    PMID: 35658231

    Sample: serum
  7. Cells (2022) IF: 6.6
    Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

    DOI: 10.3390/cells11101690

    PMID: 35626727

    Sample: serum
  8. ENVIRONMENTAL POLLUTION (2020) IF: 6.793
    Perfluorooctanoic acid exposure impact a trade-off between self-maintenance and reproduction in lizards (Eremias argus) in a gender-dependent manner

    DOI: 10.1016/j.envpol.2020.114341

    Sample: Tissue homogenate
  9. LIFE SCIENCES (2022) IF: 5.037
    Studies on testicular ultrastructural and hormonal changes in type-2 diabetic rats treated with highly active antiretroviral therapy conjugated silver nanoparticles

    DOI: 10.1016/j.lfs.2022.120498

    PMID: 35341824

    Sample: serum
  10. LIFE SCIENCES (2021) IF: 5.037
    Evaluation of maternal high-fat diet and Quercetin-3-O-rutinoside treatment on the reproductive profile of diet naïve male offspring

    DOI: 10.1016/j.lfs.2021.119179

    PMID: 33577849

    Sample: Tissue homogenate
  11. CHEMOSPHERE (2019) IF: 5.108
    T-2 toxin impairs male fertility by disrupting hypothalamic-pituitary-testis axis and declining testicular function in mice

    DOI: 10.1016/j.chemosphere.2019.06.145

    PMID: 31519099

    Sample: Serum
  12. SCIENCE OF THE TOTAL ENVIRONMENT (2019) IF: 5.589
    Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus

    DOI: 10.1016/j.scitotenv.2019.02.419

    PMID: 30833257

    Sample: Plasma
  13. ENVIRONMENTAL POLLUTION (2019) IF: 5.714
    Enrichment of imidacloprid and its metabolites in lizards and its toxic effects on gonads

    DOI: 10.1016/j.envpol.2019.113748

    PMID: 31874432

    Sample: Plasma
  14. TOXICOLOGY (2021) IF: 4.221
    Environmentally relevant perinatal exposure to DBP disturbs testicular development and puberty onset in male mice

    DOI: 10.1016/j.tox.2021.152860

    PMID: 34280466

    Sample: Serum
  15. TOXICOLOGY LETTERS (2021) IF: 4.374
    Effects of prenatal chlorocholine chloride exposure on pubertal development and reproduction of male offspring in rats

    DOI: 10.1016/j.toxlet.2021.08.005

    PMID: 34411681

    Sample: serum
  16. BRAIN RESEARCH (2018) IF: 3.125
    Intracerebroventricular injection of histamine induces the hypothalamic-pituitary-gonadal axis activation in male rats

    DOI: 10.1016/j.brainres.2018.08.020

    Sample: plasma
  17. REPRODUCTIVE TOXICOLOGY (2021) IF: 3.143
    Repression of HDAC5 by acetate restores hypothalamic-pituitary-ovarian function in type 2 diabetes mellitus

    DOI: 10.1016/j.reprotox.2021.10.008

    PMID: 34656705

    Sample: plasma
  18. Brain Sciences (2022) IF: 3.333
    Exogenous Melatonin Regulates Puberty and the Hypothalamic GnRH-GnIH System in Female Mice

    DOI: 10.3390/brainsci12111550

    Sample: serum
  19. TOXICOLOGY LETTERS (2019) IF: 3.499
    Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats

    DOI: 10.1016/j.toxlet.2019.10.024

    PMID: 31689472

    Sample: Serum
  20. FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775
    Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats

    DOI: 10.1016/j.fct.2019.110897

    PMID: 31654709

    Sample: Serum
  21. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    Cannabinoid-deficient Benin republic hemp ( Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

    DOI: 10.1186/s12906-019-2541-5

    Sample: plasma
  22. Veterinary Research Forum (2021) IF: 1.054
    Season influence on serum kisspeptin level and its association with hormonal levels and semen kinematics of buffalo bulls (Bubalus bubalis)

    DOI: 10.30466/vrf.2020.122142.2873

    Sample: serum
  23. AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL (2015) IF: 1.562
    Brain thromboxane A2 via arachidonic acid cascade induces the hypothalamic–pituitary–gonadal axis activation in rats

    DOI: 10.1016/j.autneu.2015.02.005

    Sample: Plasma
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

A***********hSubmitted [ Jun 13 2023 ]

Asked: I would like a quote for this item, if you have a bovine GnRH kit, it is most appropriate for our assay GnRH(Gonadotropin Releasing Hormone) ELISA Kit Cat.No.:E-EL-0071 Also I would like to ask for a quote for GnRH-HRP conjugate detection reagent separately, Could you provide that separately please?

Reply

A

adminSubmitted [ Jun 13 2023 ]

Answered: Hello Ahmed, I will reply you via email later.

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