Current Position:Home>>Kits>>ELISA Kits>> Human Aβ1-40(Amyloid Beta 1-40) ELISA Kit
Cat.No.:E-EL-H0542
Welcome to order from local distributors.
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human Aβ1-40 in samples. No significant cross-reactivity or interference between Human Aβ1-40 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human Aβ1-40 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Aβ1-40 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Aβ1-40 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 50.60 | 152.10 | 454.70 | 45.80 | 156.80 | 436.80 |
Standard deviation | 3.30 | 6.10 | 23.60 | 2.60 | 9.10 | 13.10 |
CV (%) | 6.52 | 4.01 | 5.19 | 5.68 | 5.80 | 3.00 |
The recovery of Human Aβ1-40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 93-109 | 101 |
EDTA plasma (n=8) | 85-96 | 91 |
Cell culture media (n=8) | 92-108 | 100 |
Samples were spiked with high concentrations of Human Aβ1-40 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P05067
|
Synonyms | Abeta 40, amyloid beta 40,Beta-amyloid protein 40,Aβ(1-40) ,Aβ40,Amyloid Beta 40 |
Research Area | Cancer, Neuroscience |
![]() |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
![]() |
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
![]() |
3. Aspirate and wash the plate for 3 times |
![]() |
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
![]() |
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
![]() |
6. Add 50μL Stop Solution |
![]() |
7. Read the plate at 450nm immediately. Calculation of the results |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!
PMID: 31978364 |
Sample: Cell culture supernatant |
PMID: 34977433 |
Sample: HT22 cell |
PMID: 30898012 |
Sample: Tissue homogenate,Cell culture supernatant,Cell ly |
Sample: hippocampal tissues |
DOI: 10.1111/acel.13469 |
|
PMID: 34528736 |
Sample: plasma |
Sample: Cell lysate |
Sample: brain |
DOI: 10.1111/tra.12874 |
|
Sample: serum |
Sample: plasma |
PMID: 35661666 |
Sample: hippocampus tissue |
DOI: 10.1111/jcpe.13267 |
|
Sample: Serum |
Sample: SH-SY5Y cell |
PMID: 32084452 |
Sample: Cell lysate |
Sample: HEK-293T cells |
Sample: hippocampus |
DOI: 10.1002/JPER.18-0560 |
|
PMID: 30417380 |
Sample: Serum |
PMID: 34015662 |
Sample: Plasma |
Sample: Cell culture medium |
PMID: 30771155 |
Sample: Tissue homogenate,Cell culture medium |
PMID: 28417423 |
Sample: Cell culture supernatant |
D******************ASubmitted [ May 03 2023 ]
Asked: Hi, I am going to start ELISA using this kit for my extracted protein samples. I have 2 questions to ask. The first question is what is the storage condition of diluted wash buffer? Can I store the diluted wash buffer in room temperature or it should be stored at 4 degree Celsius for optimum function. My second question is, the manual states addition of Stop Solution follows the same order as the addition of Substrate Reagent. What does the order means?
Reply
adminSubmitted [ May 03 2023 ]
Answered: Dear Danesh, thanks for reaching out! I'll reply to you by email. Pls pay attention to it.
D******************ASubmitted [ May 03 2022 ]
Asked: Hi, I am planning to buy this kit (E-EL-H0542) for amyloid-beta 1-40 detection from my fibroblast cell culture. The term cell-culture media used in this manual refers to the supernatant culture media, right? And to double confirm, we cannot use cell lysate for this kit, right? I hope you will help me with the proper sample method I can use along with this kit.
Reply
adminSubmitted [ May 03 2022 ]
Answered: Hi DANESH, Good morning~ "The term cell-culture media used in this manual refers to the supernatant culture media, right?" -yes, correct "And to double confirm, we cannot use cell lysate for this kit, right?" -this kit can detect Cell lysates as well as Cell culture supernatant. Any inquiry, pls feel free to contact me Thank you