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Human Aβ1-40(Amyloid Beta 1-40) ELISA Kit

Citations(21)Uniprot : P05067

Cat.No.:E-EL-H0542
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human Aβ1-40 in samples. No significant cross-reactivity or interference between Human Aβ1-40 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human Aβ1-40 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Aβ1-40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Aβ1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Aβ1-40. You can calculate the concentration of Human Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Aβ1-40 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Aβ1-40 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	50.60	152.10	454.70	45.80	156.80	436.80
Standard deviation	3.30	6.10	23.60	2.60	9.10	13.10
CV (%)	6.52	4.01	5.19	5.68	5.80	3.00

Recovery

The recovery of Human Aβ1-40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-109	101
EDTA plasma (n=8)	85-96	91
Cell culture media (n=8)	92-108	100

Linearity

Samples were spiked with high concentrations of Human Aβ1-40 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05067
Synonyms	Abeta 40, amyloid beta 40,Beta-amyloid protein 40,Aβ(1-40) ,Aβ40,Amyloid Beta 40
Research Area	Cancer, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Cell Stem Cell (2020) IF: 20.86

Interneuron Accumulation of Phosphorylated tau Impairs Adult Hippocampal Neurogenesis by Suppressing GABAergic Transmission

DOI: 10.1016/j.stem.2019.12.015
PMID: 31978364	Sample: Cell culture supernatant

Bioactive Materials (2021) IF: 14.593

Modulating autophagic flux via ROS-responsive targeted micelles to restore neuronal proteostasis in Alzheimer's disease

DOI: 10.1016/j.bioactmat.2021.09.017
PMID: 34977433	Sample: HT22 cell

Autophagy (2019) IF: 11.059

Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model

DOI: 10.1080/15548627.2019.1596488
PMID: 30898012	Sample: Tissue homogenate,Cell culture supernatant,Cell ly

JOURNAL OF CONTROLLED RELEASE (2023) IF: 11.467

Brain-neuron targeted nanoparticles for peptide synergy therapy at dual-target of Alzheimer's disease

DOI: 10.1016/j.jconrel.2023.01.074
	Sample: hippocampal tissues

AGING CELL (2021) IF: 9.304

Platelet biomarkers identifying mild cognitive impairment in type 2 diabetes patients

DOI: 10.1111/acel.13469
PMID: 34528736	Sample: plasma

Cell Reports (2018) IF: 8.032

CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer’s Disease

DOI: 10.1016/j.celrep.2018.06.009
	Sample: Cell lysate

Journal of Neuroinflammation (2022) IF: 8.3219

Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer’s disease-like mice

DOI: 10.1186/s12974-022-02521-y
	Sample: brain

TRAFFIC (2022) IF: 6.144

AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice

DOI: 10.1111/tra.12874

ANALYTICAL CHEMISTRY (2019) IF: 6.35

Glycopeptide Nanofiber Platform for Aβ-Sialic Acid Interaction Analysis and Highly Sensitive Detection of Aβ

DOI: 10.1021/acs.analchem.9b00377
	Sample: serum

Frontiers in Cell and Developmental Biology (2021) IF: 6.684

Periphery Biomarkers for Objective Diagnosis of Cognitive Decline in Type 2 Diabetes Patients

DOI: 10.3389/fcell.2021.752753
	Sample: plasma

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953

A monomeric polysaccharide from Polygonatum sibiricum improves cognitive functions in a model of Alzheimer's disease by reshaping the gut microbiota

DOI: 10.1016/j.ijbiomac.2022.05.185
PMID: 35661666	Sample: hippocampus tissue

JOURNAL OF CLINICAL PERIODONTOLOGY (2020) IF: 5.241

Periodontitis and systemic markers of neurodegeneration: A case–control study

DOI: 10.1111/jcpe.13267
	Sample: Serum

Frontiers in Cellular Neuroscience (2022) IF: 5.505

Alpha- and Gamma-Tocopherol Modulates the Amyloidogenic Pathway of Amyloid Precursor Protein in an in vitro Model of Alzheimer’s Disease: A Transcriptional Study

DOI: 10.3389/fncel.2022.846459
	Sample: SH-SY5Y cell

EXPERIMENTAL NEUROLOGY (2020) IF: 4.691

Purified oleocanthal and ligstroside protect against mitochondrial dysfunction in models of early Alzheimer's disease and brain ageing

DOI: 10.1016/j.expneurol.2020.113248
PMID: 32084452	Sample: Cell lysate

Current Alzheimer Research (2019) IF: 3.271

Genistein Decreases APP/tau Phosphorylation and Ameliorates Aβ Overproduction Through Inhibiting CIP2A

DOI: 10.2174/1567205016666190830113420
	Sample: HEK-293T cells

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023) IF: 3.322

The mitochondria-targeted small molecule SS31 delays progression of behavioral deficits by attenuating β-amyloid plaque formation and mitochondrial/synaptic deterioration in APP/PS1 mice

DOI: 10.1016/j.bbrc.2023.02.076
	Sample: hippocampus

JOURNAL OF PERIODONTOLOGY (2018) IF: 3.392

Periodontitis is associated with systemic inflammation and vascular endothelial dysfunction in patients with lacunar infarct

DOI: 10.1002/JPER.18-0560
PMID: 30417380	Sample: Serum

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849

Influence of manganese exposure on cognitive function, plasma APP and Aβ levels in older men

DOI: 10.1016/j.jtemb.2021.126788
PMID: 34015662	Sample: Plasma

Scientific Reports (2020) IF: 3.998

Comparative Effects of Alpha- and Gamma-Tocopherol on Mitochondrial Functions in Alzheimer’s Disease In Vitro Model

DOI: 10.1038/s41598-020-65570-4
	Sample: Cell culture medium

NEUROCHEMICAL RESEARCH (2019) IF: 2.782

Resveratrol Abrogates Hypoxia-Induced Up-Regulation of Exosomal Amyloid-β Partially by Inhibiting CD147

DOI: 10.1007/s11064-019-02742-3
PMID: 30771155	Sample: Tissue homogenate,Cell culture medium

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2017) IF: 1.751

Evaluation of the Expression of Amyloid Precursor Protein and the Ratio of Secreted Amyloid Beta 42 to Amyloid Beta 40 in SH-SY5Y Cells Stably Transfected with Wild-Type, Single-Mutant and Double-Mutant Forms of the APP Gene for the Study of Alzheimer’s D

DOI: 10.1007/s12010-017-2468-6
PMID: 28417423	Sample: Cell culture supernatant

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Reviews/Q&A

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Q&A

D******************ASubmitted [ May 03 2023 ]

Asked: Hi, I am going to start ELISA using this kit for my extracted protein samples. I have 2 questions to ask. The first question is what is the storage condition of diluted wash buffer? Can I store the diluted wash buffer in room temperature or it should be stored at 4 degree Celsius for optimum function. My second question is, the manual states addition of Stop Solution follows the same order as the addition of Substrate Reagent. What does the order means?

adminSubmitted [ May 03 2023 ]

Answered: Dear Danesh, thanks for reaching out! I'll reply to you by email. Pls pay attention to it.

D******************ASubmitted [ May 03 2022 ]

Asked: Hi, I am planning to buy this kit (E-EL-H0542) for amyloid-beta 1-40 detection from my fibroblast cell culture. The term cell-culture media used in this manual refers to the supernatant culture media, right? And to double confirm, we cannot use cell lysate for this kit, right? I hope you will help me with the proper sample method I can use along with this kit.

adminSubmitted [ May 03 2022 ]

Answered: Hi DANESH, Good morning~ "The term cell-culture media used in this manual refers to the supernatant culture media, right?" -yes, correct "And to double confirm, we cannot use cell lysate for this kit, right?" -this kit can detect Cell lysates as well as Cell culture supernatant. Any inquiry, pls feel free to contact me Thank you

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