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Human Aβ1-42(Amyloid Beta 1-42) ELISA Kit

Citations(29)Uniprot : P05067

Cat.No.:E-EL-H0543
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human Aβ1-42 in samples. No significant cross-reactivity or interference between Human Aβ1-42 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human Aβ1-42 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Aβ1-42. You can calculate the concentration of Human Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Aβ1-42 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Aβ1-42 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	49.20	91.80	423.30	46.70	85.70	451.40
Standard deviation	2.80	4.80	21.20	2.90	4.40	14.90
CV (%)	5.69	5.23	5.01	6.21	5.13	3.30

Recovery

The recovery of Human Aβ1-42 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-102	93
EDTA plasma (n=8)	90-103	95
Cell culture media (n=8)	85-99	91

Linearity

Samples were spiked with high concentrations of Human Aβ1-42 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05067
Synonyms	Aβ(1-42) ,Amyloid Beta 42,Amyloid Beta 42
Research Area	Cancer, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Cell Stem Cell (2020) IF: 20.86

Interneuron Accumulation of Phosphorylated tau Impairs Adult Hippocampal Neurogenesis by Suppressing GABAergic Transmission

DOI: 10.1016/j.stem.2019.12.015
PMID: 31978364	Sample: Cell culture supernatant

Bioactive Materials (2021) IF: 14.593

Modulating autophagic flux via ROS-responsive targeted micelles to restore neuronal proteostasis in Alzheimer's disease

DOI: 10.1016/j.bioactmat.2021.09.017
PMID: 34977433	Sample: HT22 cell

Alzheimers & Dementia (2018) IF: 12.764

CDT2-controlled cell cycle reentry regulates the pathogenesis of Alzheimer's disease

DOI: 10.1016/j.jalz.2018.08.013
PMID: 30321504	Sample: Cell culture medium

Autophagy (2019) IF: 11.059

Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model

DOI: 10.1080/15548627.2019.1596488
PMID: 30898012	Sample: Tissue homogenate,Cell culture supernatant,Cell ly

JOURNAL OF CONTROLLED RELEASE (2023) IF: 11.467

Brain-neuron targeted nanoparticles for peptide synergy therapy at dual-target of Alzheimer's disease

DOI: 10.1016/j.jconrel.2023.01.074
	Sample: hippocampal tissues

FOOD CHEMISTRY (2023) IF: 9.231

Enhancing the stability, BBB permeability and neuroprotective activity of verbascoside in vitro using lipid nanocapsules in combination with menthol

DOI: 10.1016/j.foodchem.2023.135682
	Sample: Human brain microvascular endothelial cells (hBMEC

AGING CELL (2021) IF: 9.304

Platelet biomarkers identifying mild cognitive impairment in type 2 diabetes patients

DOI: 10.1111/acel.13469
PMID: 34528736	Sample: plasma

Journal of Neuroinflammation (2022) IF: 8.3219

Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer’s disease-like mice

DOI: 10.1186/s12974-022-02521-y
	Sample: brain

TRAFFIC (2022) IF: 6.144

AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice

DOI: 10.1111/tra.12874

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208

New Monoterpenoid Indole Alkaloids from Tabernaemontana crassa Inhibit β-Amyloid42 Production and Phospho-Tau (Thr217)

DOI: 10.3390/ijms24021487
	Sample: U251-APP cell

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208

dNAGLU Extends Life Span and Promotes Fitness and Stress Resistance in Drosophila

DOI: 10.3390/ijms232214433

Frontiers in Cell and Developmental Biology (2021) IF: 6.684

Periphery Biomarkers for Objective Diagnosis of Cognitive Decline in Type 2 Diabetes Patients

DOI: 10.3389/fcell.2021.752753
	Sample: plasma

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953

A monomeric polysaccharide from Polygonatum sibiricum improves cognitive functions in a model of Alzheimer's disease by reshaping the gut microbiota

DOI: 10.1016/j.ijbiomac.2022.05.185
PMID: 35661666	Sample: hippocampus tissue

JOURNAL OF CLINICAL PERIODONTOLOGY (2020) IF: 5.241

Periodontitis and systemic markers of neurodegeneration: A case–control study

DOI: 10.1111/jcpe.13267
	Sample: Serum

NEUROPHARMACOLOGY (2021) IF: 5.251

Low molecular weight chondroitin sulfate ameliorates pathological changes in 5XFAD mice by improving various functions in the brain

DOI: 10.1016/j.neuropharm.2021.108796
PMID: 34543632	Sample: brain

Organic Chemistry Frontiers (2022) IF: 5.281

(±)-Spiroganoapplanin A, a complex polycyclic meroterpenoid dimer from Ganoderma applanatum displaying potential against Alzheimer's disease

DOI: 10.1039/D2QO00246A
	Sample: U251-APP cell

Frontiers in Cellular Neuroscience (2022) IF: 5.505

Alpha- and Gamma-Tocopherol Modulates the Amyloidogenic Pathway of Amyloid Precursor Protein in an in vitro Model of Alzheimer’s Disease: A Transcriptional Study

DOI: 10.3389/fncel.2022.846459
	Sample: SH-SY5Y cell

Translational Neurodegeneration (2019) IF: 5.534

Enriched gestation activates the IGF pathway to evoke embryo-adult benefits to prevent Alzheimer’s disease

DOI: 10.1186/s40035-019-0149-9
	Sample: Tissue homogenate

Frontiers in Aging Neuroscience (2022) IF: 5.702

Heterogeneous nuclear ribonucleoprotein A2/B1 as a novel biomarker in elderly patients for the prediction of postoperative neurocognitive dysfunction: A prospective nested case-control study.

DOI: 10.3389/fnagi.2022.1034041
	Sample: plasma

PHYTOCHEMISTRY (2022) IF: 4.004

Monoterpenoid indole alkaloid dimers from Kopsia arborea inhibit cyclin-dependent kinase 5 and tau phosphorylation

DOI: 10.1016/j.phytochem.2022.113392
PMID: 36030903	Sample: U251-APP cell

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION (2022) IF: 4.256

Circulating levels of PIN1 and glucose metabolism in young people with obesity

DOI: 10.1007/s40618-022-01812-8
PMID: 35585295	Sample: serum

Frontiers in Aging Neuroscience (2020) IF: 4.364

Blood-Based Biomarkers for Predictive Diagnosis of Cognitive Impairment in a Pakistani Population

DOI: 10.3389/fnagi.2020.00223
PMID: 32848704	Sample: Serum

Current Alzheimer Research (2019) IF: 3.271

Genistein Decreases APP/tau Phosphorylation and Ameliorates Aβ Overproduction Through Inhibiting CIP2A

DOI: 10.2174/1567205016666190830113420
	Sample: HEK-293T cells

JOURNAL OF PERIODONTOLOGY (2018) IF: 3.392

Periodontitis is associated with systemic inflammation and vascular endothelial dysfunction in patients with lacunar infarct

DOI: 10.1002/JPER.18-0560
PMID: 30417380	Sample: Serum

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849

Influence of manganese exposure on cognitive function, plasma APP and Aβ levels in older men

DOI: 10.1016/j.jtemb.2021.126788
PMID: 34015662	Sample: Plasma

Scientific Reports (2020) IF: 3.998

Comparative Effects of Alpha- and Gamma-Tocopherol on Mitochondrial Functions in Alzheimer’s Disease In Vitro Model

DOI: 10.1038/s41598-020-65570-4
	Sample: Cell culture medium

BMC Veterinary Research (2021) IF: 2.741

Change in the plasma proteome associated with canine cognitive dysfunction syndrome (CCDS) in Thailand

DOI: 10.1186/s12917-021-02744-w
PMID: 33514370	Sample: Plasma

NEUROCHEMICAL RESEARCH (2019) IF: 2.782

Resveratrol Abrogates Hypoxia-Induced Up-Regulation of Exosomal Amyloid-β Partially by Inhibiting CD147

DOI: 10.1007/s11064-019-02742-3
PMID: 30771155	Sample: Tissue homogenate,Cell culture medium

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2017) IF: 1.751

Evaluation of the Expression of Amyloid Precursor Protein and the Ratio of Secreted Amyloid Beta 42 to Amyloid Beta 40 in SH-SY5Y Cells Stably Transfected with Wild-Type, Single-Mutant and Double-Mutant Forms of the APP Gene for the Study of Alzheimer’s D

DOI: 10.1007/s12010-017-2468-6
PMID: 28417423	Sample: Cell culture supernatant

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Reviews/Q&A

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Reviews
Q&A

S*********nSubmitted [ Jul 13 2023 ]

Asked: Hello, I had an e-mail about your answer but I couldn’t see the answer at the Q/A part for my belowed question: “I have a question about the usage of this kit in amiloid-beta 1-42 toxicity. We will make a toxicity model of amyloid-beta 1-42 peptides by incubating for fibrillation and then adding them to the cell. Next, we\\\'ll look at how a drug affects the release of amyloid-beta 1-42 from the cell in this model. Does this kit interact with fibrillated amyloid 1-42? And if there are peptides left without fibrillation in the medium, do they cause wrong result with those released amyloid-beta 1-42 from the cell? I would be very happy if you could help.” Thank you for your support.

C*****eSubmitted [ Jul 13 2023 ]

Answered: We are sorry that we have not verified the cell supernatant at present, and have not done toxicity verification, so we are not sure about the influence of toxic drugs on the cell culture medium. It is recommended to do a preliminary experiment to determine the applicability

S*********nSubmitted [ Jul 13 2023 ]

Asked: I have a question about the usage of this kit in amiloid-beta 1-42 toxicity. We will make a toxicity model of amyloid-beta 1-42 peptides by incubating for fibrillation and then adding them to the cell. Next, we\'ll look at how a drug affects the release of amyloid-beta 1-42 from the cell in this model. Does this kit interact with fibrillated amyloid 1-42? And if there are peptides left without fibrillation in the medium, do they cause wrong result with those released amyloid-beta 1-42 from the cell? I would be very happy if you could help.

C*****eSubmitted [ Jul 13 2023 ]

Answered: E-EL-H0543 Human Aβ1-42(Amyloid Beta 1-42) ELISA Kit For E-EL-H0543 kit, we have not verified the cell culture medium, and since the concentration of the target protein varies with the number of cells, growth status, intervention conditions, etc., it is recommended to conduct preliminary experiments referring to the related literatures to confirm its effectiveness. In addition, the kit could specifically identifies human Aβ1-42 in serum/plasma samples. We are willing to provide customers with a 24T free kit

C*****eSubmitted [ Jul 13 2023 ]

adminSubmitted [ Jul 13 2023 ]

A*************rSubmitted [ May 22 2023 ]

Asked: Could u please tell me how to store saliva for abeta 42 for around until analysis which will be after 7 months

adminSubmitted [ May 22 2023 ]

Answered: Hello Ashwini S kumar,thank you for your support.Under normal circumstances, it is recommended to store saliva samples at -80 ° C for less than 3 months. Therefore we cannot guarantee the accuracy of samples measured after 7 months, so we suggest customers to prepare fresh samples for testing.

D******************ASubmitted [ May 12 2023 ]

Asked: I have a question to as about the Amyloid beta detected using this ELISA kit. What is the form of Amyloid beta 42 that is detected from cell lysate versus cell culture supernatant from cell culture? Does the AB42 detected from cell culture supernatant means soluble form of Amyloid Beta 42 and from the cell lysates are Amyloid Beta 42 that internalized in the cells? Hope can get some clarifications from the product specialist. Feel free to suggest any literatures/papers for a better understanding about AB42 detected from cell lysates and culture supernatant. Thank you

adminSubmitted [ May 12 2023 ]

Answered: Hi Danesh, thanks for reaching out. I'll reply to you by email. Pls pay attention to it.

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Cat. No	E-EL-H0543
Species

Human Aβ1-42(Amyloid Beta 1-42) ELISA Kit

To Purchase E-EL-H0543

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

Cell Stem Cell (2020) IF: 20.86

Bioactive Materials (2021) IF: 14.593

Alzheimers & Dementia (2018) IF: 12.764

Autophagy (2019) IF: 11.059

JOURNAL OF CONTROLLED RELEASE (2023) IF: 11.467

FOOD CHEMISTRY (2023) IF: 9.231

AGING CELL (2021) IF: 9.304

Journal of Neuroinflammation (2022) IF: 8.3219

TRAFFIC (2022) IF: 6.144

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208

Frontiers in Cell and Developmental Biology (2021) IF: 6.684

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953

JOURNAL OF CLINICAL PERIODONTOLOGY (2020) IF: 5.241

NEUROPHARMACOLOGY (2021) IF: 5.251

Organic Chemistry Frontiers (2022) IF: 5.281

Frontiers in Cellular Neuroscience (2022) IF: 5.505

Translational Neurodegeneration (2019) IF: 5.534

Frontiers in Aging Neuroscience (2022) IF: 5.702

PHYTOCHEMISTRY (2022) IF: 4.004

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION (2022) IF: 4.256

Frontiers in Aging Neuroscience (2020) IF: 4.364

Current Alzheimer Research (2019) IF: 3.271

JOURNAL OF PERIODONTOLOGY (2018) IF: 3.392

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849

Scientific Reports (2020) IF: 3.998

BMC Veterinary Research (2021) IF: 2.741

NEUROCHEMICAL RESEARCH (2019) IF: 2.782

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2017) IF: 1.751

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