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Cat.No.:E-EL-H0137
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of ACTH concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ACTH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ACTH were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 45.70 | 130.90 | 461.60 | 45.10 | 124.70 | 448.60 |
Standard deviation | 3.20 | 6.80 | 14.30 | 2.30 | 6.40 | 19.70 |
CV (%) | 7.00 | 5.19 | 3.10 | 5.10 | 5.13 | 4.39 |
The recovery of ACTH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 91-104 | 98 |
EDTA plasma(n=8) | 85-100 | 91 |
Cell culture media(n=8) | 86-98 | 93 |
Samples were spiked with high concentrations of Human ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Corticotropin, Adrenocorticotropin |
Research Area | Cancer, Metabolism, Neuroscience, Signal transduction |
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1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
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PMID: 35870527 |
Sample: serum |
PMID: 33304464 |
Sample: blood |
DOI: 10.3390/nu13041321 |
|
PMID: 33923663 |
Sample: Whole Blood |
I****rSubmitted [ Aug 20 2018 ]
Asked: Good Morning !! I need to know if this kit (E-EL-H0137) can be used (validated) with saliva samples. So if you can send the options to me I appreciate it. Thank you
Reply
adminSubmitted [ Aug 20 2018 ]
Answered: Thank you Itamar for your question. For this kit with Cat. #E-EL-H0137, it can be used with saliva samples. Please feel free to contact us if any other question. Thank you.