|Detection range||7.81-500 pg/mL|
|Sample type &Sample volume||serum, plasma and other biological fluids; 50μL|
|Specificity||This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed.|
|Reproducibility||Both intra-CV and inter-CV are < 10%.|
|Application||This ELISA kit applies to the in vitro quantitative determination of ANP concentrations in serum, plasma and other biological fluids.|
|Micro ELISA Plate(Dismountable)||96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
|-20℃, 6 months|
|Reference Standard||96T: 2 vials
48T: 1 vial
|Concentrated Biotinylated Detection Ab (100×)||96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
|Concentrated HRP Conjugate (100×)||96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
|-20℃(Protect from light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||2-8°C, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25×)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||2-8℃(Protect from light)|
|Stop Solution||1 vial, 10 mL||2-8℃|
|Plate Sealer||5 pieces|
|Certificate of Analysis||1 copy|
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ANP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ANP were tested on 3 different plates, 20 replicates in each plate.
|Intra-assay Precision||Inter-assay Precision|
The recovery of ANP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
|Sample Type||Range (%)||Average Recovery (%)|
|Cell culture media(n=8)||92-107||99|
Samples were spiked with high concentrations of Human ANP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
|Database Links||SwissProt: P01160|
|Synonyms||NPPA, ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND, Atrial natriuretic peptide, natriuretic peptide A|
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results
|E-ELIR-001||ELISA Plate Coating Buffer(1×)||100mL, 1000mL|
|E-ELIR-002||ELISA Plate Coating Buffer(5×)||100mL, 1000mL|
|E-ELIR-003||ELISA Plate Blocking Buffer||100mL, 1000mL|
|E-ELIR-004||Wash Buffer for Sandwich-ELISA(25×)||100mL, 1000mL|
|E-ELIR-005||Wash Buffer for Competitive-ELISA(25×)||100mL, 1000mL|
|E-ELIR-006||Stop Solution||100mL, 1000mL|
|E-ELIR-007||HRP-conjugate Stabilizer||10mL, 100mL, 500mL|
|E-ELIR-008||HRP-conjugate Diluent||100mL, 1000mL|
|E-ELIR-009||Biotinylated Antibody Stabilizer||10mL, 100mL, 500mL|
|E-ELIR-0010||Biotinylated Antibody Diluent||10mL, 100mL, 500mL|
|E-ELIR-0011||Sample Diluent||10mL, 100mL, 500mL|
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M**********nSubmitted [ Jun 15 2018 ]
Asked: Does this kit cross-react with mouse ANP?
adminSubmitted [ Jun 15 2018 ]
Answered: Hi Marina, Thanks for your inquiry. The cross-reactivity with the mouse ANP is 22.4% in our lab as the identity of the immunogen sequences is high. Any other questions, pls feel free to contact us. Best regards.