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Human APLN(Apelin) ELISA Kit

  • Cat.No.:E-EL-H0456

  • Reactivity: Human

To Purchase E-EL-H0456

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes APLN in samples. No significant cross-reactivity or interference between APLN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of APLN concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with APLN. During the reaction, APLN in the sample or standard competes with a fixed amount of APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level APLN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level APLN were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 197.40 537.10 1359.90 204.30 499.70 1350.70
Standard deviation 11.10 31.70 50.30 11.00 26.00 56.70
CV (%) 5.62 5.90 3.70 5.38 5.20 4.20

Recovery

The recovery of APLN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 90
EDTA plasma(n=8) 91-102 96
Cell culture media(n=8) 89-100 95

Linearity

Samples were spiked with high concentrations of Human APLN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q9ULZ1
SynonymsAPEL, XNPEP2
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Biomedicines (2022) IF: 4.757
    The Effect of SGLT2 Inhibitor Dapagliflozin on Serum Levels of Apelin in T2DM Patients with Heart Failure

    DOI: 10.3390/biomedicines10071751

    PMID: 35885056

    Sample: Serum
  2. Frontiers in Pharmacology (2022) IF: 5.988
    Elabela Attenuates the TGF-β1-Induced Epithelial-Mesenchymal Transition of Peritoneal Mesothelial Cells in Patients Receiving Peritoneal Dialysis

    DOI: 10.3389/fphar.2022.890881

    PMID: 35800446

    Sample: Serum
  3. Gene Reports (2021)
    Association of Apelin gene rs3115757 variation and expression with metabolic syndrome among female students in King Abdulaziz University

    DOI: 10.1016/j.genrep.2021.101305

    Sample: Serum
  4. Indian Journal of Surgery (2020) IF: 0.561
    Serum Apelin Levels and Ascending Aortic Aneurysm in Adults

    DOI: 10.1007/s12262-020-02440-3

    Sample: Serum
  5. THERAPEUTIC APHERESIS AND DIALYSIS (2016) IF: 1.477
    Evaluation of Fibrosis Markers: Apelin and Transforming Growth Factor‐β1 in Autosomal Dominant Polycystic Kidney Disease Patients

    DOI: 10.1111/1744-9987.12412

    Sample: Serum
  6. MEDICINE (2021) IF: 1.889
    Association of serum vaspin, apelin, and visfatin levels and stroke risk in a Chinese case-control study

    DOI: 10.1097/MD.0000000000025184

    PMID: 33761698

    Sample: Serum
  7. DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
    Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

    DOI: 10.1016/j.diabres.2019.05.023

    PMID: 31150725

    Sample: Serum
  8. Journal of Cardiovascular Development and Disease (2022) IF: 3.948
    Discriminative Utility of Apelin-to-NT-Pro-Brain Natriuretic Peptide Ratio for Heart Failure with Preserved Ejection Fraction among Type 2 Diabetes Mellitus Patients

    DOI: 10.3390/jcdd9010023

    Sample: Plasma
  9. Diagnostics (2022) IF: 3.992
    Interplay between Myokine Profile and Glycemic Control in Type 2 Diabetes Mellitus Patients with Heart Failure

    DOI: 10.3390/diagnostics12122940

    Sample: Serum
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