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Human APLN(Apelin) ELISA Kit

  • Cat.No.:E-EL-H0456

  • Reactivity: Human

To Purchase E-EL-H0456

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes APLN in samples. No significant cross-reactivity or interference between APLN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of APLN concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with APLN. During the reaction, APLN in the sample or standard competes with a fixed amount of APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level APLN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level APLN were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 197.40 537.10 1359.90 204.30 499.70 1350.70
Standard deviation 11.10 31.70 50.30 11.00 26.00 56.70
CV (%) 5.62 5.90 3.70 5.38 5.20 4.20

Recovery

The recovery of APLN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 90
EDTA plasma(n=8) 91-102 96
Cell culture media(n=8) 89-100 95

Linearity

Samples were spiked with high concentrations of Human APLN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q9ULZ1
SynonymsAPEL, XNPEP2

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Diabetes research and clinical practice (2019) IF: 4.234
    Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

    DOI: 10.1016/j.diabres.2019.05.023

    PMID: 31150725

    Sample: Serum
  2. Journal of Functional Foods (2020) IF: 3.701
    Serum Apelin Levels and Ascending Aortic Aneurysm in Adults

    DOI: 10.1007/s12262-020-02440-3

    PMID: 99999999

    Sample: Serum
  3. Therapeutic Apheresis and Dialysis (2016) IF: 1.529
    Evaluation Of Fibrosis Markers: Apelin And Transforming Growth Factor-Β1 In Autosomal Dominant Polycystic Kidney Disease Patients.

    DOI: 10.1111/1744-9987.12412

    PMID: 26991810

    Sample: Serum
  4. Open access Macedonian journal of medical sciences (2018)
    Serum Apelin: A New Marker of Early Atherosclerosis in Children with Type 1 Diabetes Mellitus

    DOI: 10.3889/oamjms.2018.144

    PMID: 29731925

    Sample: Serum
  5. Open access Macedonian journal of medical sciences (2018)
    Serum Apelin and Obesity-Related Complications in Egyptian Children

    DOI: 10.3889/oamjms.2018.312

    PMID: 30159056

    Sample: Serum
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