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Human ApoA4(Apolipoprotein A4) ELISA Kit

  • Cat.No.:E-EL-H0463

  • Reactivity: Human

To Purchase E-EL-H0463

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 4.69-300 ng/mL
Sensitivity 2.81 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human ApoA4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoA4. You can calculate the concentration of Human ApoA4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ApoA4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ApoA4 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 14.00 45.80 115.80 12.70 47.40 118.00
Standard deviation 0.90 2.50 3.90 0.80 2.50 5.80
CV (%) 6.43 5.46 3.37 6.30 5.27 4.92

Recovery

The recovery of Human ApoA4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 95
EDTA plasma (n=8) 88-102 95
Cell culture media (n=8) 90-103 95

Linearity

Samples were spiked with high concentrations of Human ApoA4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P06727
SynonymsAPOA4,Apolipoprotein A-IV,ApoA-IV,ApoAIV,apoA4,
Research AreaCardiovascular,Signaling transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Bioelectrochemistry (2020) IF: 4.722
    A novel electrochemical immunosensor for the highly sensitive and selective detection of the depression marker human apolipoprotein A4

    DOI: 10.1016/j.bioelechem.2020.107542

    PMID: 32388438

    Sample: Plasma
  2. Journal of Molecular and Cellular Cardiology (2020) IF: 4.133
    Identifying functional non-coding variants in APOA5/A4/C3/A1 gene cluster associated with coronary heart disease

    DOI: 10.1016/j.yjmcc.2020.05.003

    PMID: 32437778

    Sample: Serum
  3. Scientific Reports (2016) IF: 4.259
    iTRAQ-Based Proteomic Analysis Of Plasma Reveals Abnormalities In Lipid Metabolism Proteins In Chronic Kidney Disease-Related Atherosclerosis.

    DOI: 10.1038/srep32511

    PMID: 27600335

    Sample: Plasma
  4. Scientific Reports (2016) IF: 4.259
    Itraq-Based Proteomic Analysis Of Plasma Reveals Abnormalities In Lipid Metabolism Proteins In Chronic Kidney Disease-Related Atherosclerosis.

    DOI: 10.1038/srep32511

    PMID: 27600335

    Sample: Plasma
  5. International Journal of Molecular Sciences (2016) IF: 3.226
    Label-Free Quantitative Proteomics Reveals Differences In Molecular Mechanism Of Atherosclerosis Related And Non-Related To Chronic Kidney Disease.

    DOI: 10.3390/ijms17050631

    PMID: 27144566

    Sample: Plasma
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