Human AXL(AXL Receptor Tyrosine Kinase) ELISA Kit (E-EL-H2619)

Name: Human AXL(AXL Receptor Tyrosine Kinase) ELISA Kit
Brand: Elabscience
SKU: E-EL-H2619
Price: 150.0 USD
Availability: InStock

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Human AXL(AXL Receptor Tyrosine Kinase) ELISA Kit - 1

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Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Reactivity	Human
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

	OD1	OD2	Mean OD	Corrected OD
10	2.332	2.264	2.298	2.220
5	1.574	1.614	1.594	1.516
2.5	0.907	0.947	0.927	0.849
1.25	0.505	0.501	0.503	0.425
0.63	0.310	0.316	0.313	0.235
0.32	0.192	0.186	0.189	0.111
0.16	0.139	0.131	0.135	0.057
0	0.076	0.080	0.078	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human AXL were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human AXL were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（）	0.530	1.390	3.910	0.510	1.510	3.820
Standard deviation	0.040	0.090	0.210	0.030	0.120	0.320
CV（%）	6.700	6.680	5.460	6.450	7.780	8.500

Recovery

The recovery of Human AXL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	95-107	101
Serum （n=8）	85-100	91
EDTA plasma （n=8）	84-98	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AXL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.