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Human Bcl-2(B-cell Leukemia/Lymphoma 2) ELISA Kit

Citations(15)Uniprot : P10415

Cat.No.:E-EL-H0114
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human Bcl-2 in samples. No significant cross-reactivity or interference between Human Bcl-2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Bcl-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Bcl-2. You can calculate the concentration of Human Bcl-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Bcl-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Bcl-2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.60	4.20	0.50	1.60	3.80
Standard deviation	0.03	0.08	0.15	0.03	0.08	0.20
CV (%)	6.00	5.00	3.57	6.00	5.00	5.26

Recovery

The recovery of Human Bcl-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-101	96
EDTA plasma (n=8)	86-96	91
Cell culture media (n=8)	92-105	100

Linearity

Samples were spiked with high concentrations of Human Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P10415
Synonyms	Bcl2, PPP1R50
Research Area	Cancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.446
Activable Targeted Protein Degradation Platform Based on Light-triggered Singlet Oxygen

DOI: 10.1021/acs.jmedchem.1c02037

PMID: 35164509
Sample: MCF-7 cell
EBioMedicine (2019) IF: 6.68
Three kinds of corneal host cells contribute differently to corneal neovascularization

DOI: 10.1016/j.ebiom.2019.05.026

PMID: 31126890
Sample: Tissue homogenate
LIFE SCIENCES (2022) IF: 5.037
Integration of synthetic and natural derivatives revives the therapeutic potential of temozolomide against glioma- an in vitro and in vivo perspective

DOI: 10.1016/j.lfs.2022.120609

PMID: 35526592
Sample: C6 cell,U-87 MG cell,LN229 cell
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2021) IF: 5.225
Association between serum concentrations of anti-apoptotic B-cell lymphoma-2 protein and traumatic brain injury mortality

DOI: 10.1080/14737159.2022.2016394

PMID: 34878357
Sample: serum
Frontiers in Cellular and Infection Microbiology (2021) IF: 5.293
Antimicrobial Peptides Human Beta-Defensin-2 and -3 Protect the Gut During Candida albicans Infections Enhancing the Intestinal Barrier Integrity: In Vitro Study

DOI: 10.3389/fcimb.2021.666900

Sample: Caco-2 cells
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Synergistic Effect of the Long-Term Overexpression of Bcl-2 and BDNF Lentiviral in Cell Protecting against Death and Generating TH Positive and CHAT Positive Cells from MSC

DOI: 10.3390/ijms22137086

PMID: 34209365
Sample: WJ-MSC cells
MOLECULES (2022) IF: 4.927
Molecular Dereplication and In Vitro and In Silico Pharmacological Evaluation of Coriandrum sativum against Neuroblastoma Cells

DOI: 10.3390/molecules27175389

PMID: 36080159
Sample: SH-SY5Y cell
MOLECULES (2022) IF: 4.927
Dual Topoisomerase I/II Inhibition-Induced Apoptosis and Necro-Apoptosis in Cancer Cells by a Novel Ciprofloxacin Derivative via RIPK1/RIPK3/MLKL Activation

DOI: 10.3390/molecules27227993
NEUROLOGICAL SCIENCES (2021) IF: 3.307
Serum B cell lymphoma-2 concentrations and mortality of patients with spontaneous intracerebral hemorrhage

DOI: 10.1007/s10072-021-05048-x

PMID: 33433754
Sample: Serum
ACS Omega (2022) IF: 3.512
Thymoquinone Induces Nrf2 Mediated Adaptive Homeostasis: Implication for Mercuric Chloride-Induced Nephrotoxicity

DOI: 10.1021/acsomega.2c00028

PMID: 35252727
Sample: liver
JOURNAL OF MOLECULAR STRUCTURE (2022) IF: 3.841
Discovery of novel potent human chondrosarcoma (SW1353) inhibitors: 4-(2/3/4-pyridyl)thiazole 2-acetamide derivatives

DOI: 10.1016/j.molstruc.2022.134260

Sample: SW1353 cell
Journal of Stroke & Cerebrovascular Diseases (2021) IF: 2.136
Serum Levels of B-cell Lymphoma-2 Anti-Apoptotic Protein and Malignant Middle Cerebral Artery Infarction Mortality

DOI: 10.1016/j.jstrokecerebrovasdis.2021.105717

PMID: 33690027
Sample: Serum
Biomarkers in Medicine (2022) IF: 2.851
Blood Bcl-2 levels to predict the mortality of septic patients

DOI: 10.2217/bmm-2021-0226

Sample: Serum
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA (2020) IF: 1.654
Circulating Bcl-2 concentrations and septic patient mortality

DOI: 10.1016/j.eimc.2020.06.001

Sample: serum
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2019) IF: 0.439
Compound Bieshe Kang’ai inhibits proliferation and induces apoptosis in HCT116 human colorectal cancer cells

DOI: 10.4314/tjpr.v17i11.8

Sample: Cell culture supernatant

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