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Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

Citations(25)Uniprot : P23560

  • Cat.No.:E-EL-H0010

  • Reactivity: Human
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To Purchase E-EL-H0010

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human BDNF in samples. No significant cross-reactivity or interference between Human BDNF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human BDNF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BDNF. You can calculate the concentration of Human BDNF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human BDNF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human BDNF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 105.80 237.40 809.50 114.20 237.50 768.50
Standard deviation 5.80 12.80 33.20 6.70 10.00 28.40
CV (%) 5.48 5.39 4.10 5.87 4.21 3.70

Recovery

The recovery of Human BDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-109 101
EDTA plasma (n=8) 88-100 93
Cell culture media (n=8) 92-106 100

Linearity

Samples were spiked with high concentrations of Human BDNF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P23560
SynonymsANON2, BULN2
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Cell Death & Disease (2022) IF: 9.685
    LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease

    DOI: 10.1038/s41419-022-05068-1

    PMID: 35906216

    		 Sample: plasma
  2. Journal of Functional Foods (2022) IF: 5.223
    Effects of erinacine A-enriched Hericium erinaceus on elderly hearing-impaired patients: A double-blind, randomized, placebo-controlled clinical trial

    DOI: 10.1016/j.jff.2022.105220
    Sample: serum
  3. Food & Function (2022) IF: 5.396
    A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation

    DOI: 10.1039/D1FO03896F
    Sample: serum
  4. Nutrients (2021) IF: 5.719
    Effects of 12 Weeks Cosmos caudatus Supplement among Older Adults with Mild Cognitive Impairment: A Randomized, Double-Blind and Placebo-Controlled Trial

    DOI: 10.3390/nu13020434

    PMID: 33572715

    		 Sample: Serum
  5. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2022) IF: 5.785
    Sublingual buprenorphine/naloxone treatment is not affected by OPRM1 A118G and BDNF Va66Met polymorphisms, but alters the plasma beta-endorphin and BDNF levels in individuals with opioid use disorder

    DOI: 10.1016/j.etap.2022.103979

    PMID: 36174909

    		 Sample: plasma
  6. Cell Death & Disease (2019) IF: 5.959
    Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction

    DOI: 10.1038/s41419-019-1670-6
    Sample: plasm
  7. American Journal of Translational Research (2021) IF: 4.06
    Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy

    PMID: 34650715

    		 Sample: serum
  8. Stem Cell Research & Therapy (2017) IF: 4.211
    Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats

    DOI: 10.1186/s13287-017-0731-y
    Sample: human periodontal ligament-derived stem cells (hPD
  9. EUROPEAN NEUROPSYCHOPHARMACOLOGY (2022) IF: 4.6
    The role of BDNF and NGF plasma levels in first-episode schizophrenia: A longitudinal study

    DOI: 10.1016/j.euroneuro.2022.02.003

    PMID: 35219096

    		 Sample: plasma
  10. JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
    Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

    DOI: 10.1016/j.jpsychires.2022.04.002

    PMID: 35429740

    		 Sample: serum
  11. Journal of Clinical Medicine (2022) IF: 4.964
    The Effects of Four Weeks of Chiropractic Spinal Adjustments on Blood Biomarkers in Adults with Chronic Stroke: Secondary Outcomes of a Randomized Controlled Trial

    DOI: 10.3390/jcm11247493
    Sample: serum
  12. EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115
    Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damage

    DOI: 10.1111/ejn.14662
    Sample: Tissue homogenate
  13. Frontiers in Psychiatry (2019) IF: 3.161
    Longitudinal Investigation of Psychotherapy Outcomes (LIPO): Description of the Study Protocol

    DOI: 10.3389/fpsyt.2019.00212
    Sample: serum
  14. NEUROPEPTIDES (2022) IF: 3.286
    Altered levels of neurobiological biomarkers at the interface of depression and gestational diabetes mellitus in Asian Indian women

    DOI: 10.1016/j.npep.2022.102245

    PMID: 35461022

    		 Sample: plasma
  15. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849
    Association of blood lead levels with neurobehavior and BDNF expression in school going children

    DOI: 10.1016/j.jtemb.2021.126749

    PMID: 33773278

    		 Sample: Serum
  16. PEPTIDES (2022) IF: 3.867
    Interleukin-6, undercarboxylated osteocalcin, and brain-derived neurotrophic factor responses to single and repeated sessions of high-intensity interval exercise

    DOI: 10.1016/j.peptides.2022.170864

    PMID: 36028073

    		 Sample: plasma
  17. SCHIZOPHRENIA RESEARCH (2018) IF: 3.958
    Schizophrenia-associated rs4702 G allele-specific downregulation of FURIN expression by miR-338-3p reduces BDNF production

    DOI: 10.1016/j.schres.2018.02.040
    Sample: Human embryonic kidney (HEK) 293T cells
  18. NEUROUROLOGY AND URODYNAMICS (2020) IF: 2.037
    The profile of urinary biomarkers in overactive bladder

    DOI: 10.1002/nau.24487

    PMID: 32813897

    		 Sample: Urine
  19. JOURNAL OF INTELLECTUAL DISABILITY RESEARCH (2021) IF: 2.424
    Association of BDNF levels with IQ: comparison of S100B and BDNF levels in typically developing children and subjects with neurologically normal nonsyndromic intellectual disability

    DOI: 10.1111/jir.12896

    PMID: 34750906

    		 Sample: Serum
  20. BMC Complementary and Alternative Medicine (2020) IF: 2.833
    The effects of six months Persicaria minor extract supplement among older adults with mild cognitive impairment: a double-blinded, randomized, and placebo-controlled trial

    DOI: 10.1186/s12906-020-03092-2

    PMID: 33076878

    		 Sample: Serum
  21. NEUROBIOLOGY OF LEARNING AND MEMORY (2021) IF: 2.877
    Left lateral parietal rTMS improves cognition and modulates resting brain connectivity in patients with Alzheimer’s disease: Possible role of BDNF and oxidative stress

    DOI: 10.1016/j.nlm.2021.107410
    Sample: Serum
  22. Genetic Testing and Molecular Biomarkers (2021) IF: 1.795
    miR-132-3p, miR-106b-5p, and miR-19b-3p Are Associated with Brain-Derived Neurotrophic Factor Production and Clinical Activity in Multiple Sclerosis: A Pilot Study

    DOI: 10.1089/gtmb.2021.0183

    PMID: 34788141

    		 Sample: serum
  23. Sport Sciences for Health (2022)
    The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG

    DOI: 10.1007/s11332-022-00992-3
    Sample: plasma,serum
  24. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2022) IF: 0.481
    Concentrations of B cell-activating factor, aquaporin-4 antibody and brain-derived neurotrophic factor in neuromyelitis optica spectrum disorder

    DOI: 10.1515/tjb-2022-0061
    Sample: cerebrospinal fluid,serum
  25. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2022) IF: 0.533
    Dysfunctional oxidative stress response in first-episode of schizophrenia

    DOI: 10.4314/tjpr.v20i6.22
    Sample: serum
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