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Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

Citations(25)Uniprot : P23560

Cat.No.:E-EL-H0010
Reactivity: Human

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human BDNF in samples. No significant cross-reactivity or interference between Human BDNF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human BDNF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BDNF. You can calculate the concentration of Human BDNF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human BDNF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human BDNF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	105.80	237.40	809.50	114.20	237.50	768.50
Standard deviation	5.80	12.80	33.20	6.70	10.00	28.40
CV (%)	5.48	5.39	4.10	5.87	4.21	3.70

Recovery

The recovery of Human BDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-109	101
EDTA plasma (n=8)	88-100	93
Cell culture media (n=8)	92-106	100

Linearity

Samples were spiked with high concentrations of Human BDNF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P23560
Synonyms	ANON2, BULN2
Research Area	Cancer, Cardiovascular, Metabolism, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Death & Disease (2019) IF: 5.959

Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction

DOI: 10.1038/s41419-019-1670-6
	Sample: Plasm

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2022) IF: 5.785

Sublingual buprenorphine/naloxone treatment is not affected by OPRM1 A118G and BDNF Va66Met polymorphisms, but alters the plasma beta-endorphin and BDNF levels in individuals with opioid use disorder

DOI: 10.1016/j.etap.2022.103979
PMID: 36174909	Sample: Plasma

Sport Sciences for Health (2022)

The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG

DOI: 10.1007/s11332-022-00992-3
	Sample: Plasma,Serum

Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2022) IF: 0.481

Concentrations of B cell-activating factor, aquaporin-4 antibody and brain-derived neurotrophic factor in neuromyelitis optica spectrum disorder

DOI: 10.1515/tjb-2022-0061
	Sample: Cerebrospinal Fluid,Serum

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2022) IF: 0.533

Dysfunctional oxidative stress response in first-episode of schizophrenia

DOI: 10.4314/tjpr.v20i6.22
	Sample: Serum

Genetic Testing and Molecular Biomarkers (2021) IF: 1.795

miR-132-3p, miR-106b-5p, and miR-19b-3p Are Associated with Brain-Derived Neurotrophic Factor Production and Clinical Activity in Multiple Sclerosis: A Pilot Study

DOI: 10.1089/gtmb.2021.0183
PMID: 34788141	Sample: Serum

NEUROUROLOGY AND URODYNAMICS (2020) IF: 2.037

The profile of urinary biomarkers in overactive bladder

DOI: 10.1002/nau.24487
PMID: 32813897	Sample: Urine

JOURNAL OF INTELLECTUAL DISABILITY RESEARCH (2021) IF: 2.424

Association of BDNF levels with IQ: comparison of S100B and BDNF levels in typically developing children and subjects with neurologically normal nonsyndromic intellectual disability

DOI: 10.1111/jir.12896
PMID: 34750906	Sample: Serum

BMC Complementary and Alternative Medicine (2020) IF: 2.833

The effects of six months Persicaria minor extract supplement among older adults with mild cognitive impairment: a double-blinded, randomized, and placebo-controlled trial

DOI: 10.1186/s12906-020-03092-2
PMID: 33076878	Sample: Serum

NEUROBIOLOGY OF LEARNING AND MEMORY (2021) IF: 2.877

Left lateral parietal rTMS improves cognition and modulates resting brain connectivity in patients with Alzheimer’s disease: Possible role of BDNF and oxidative stress

DOI: 10.1016/j.nlm.2021.107410
	Sample: Serum

EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115

Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damage

DOI: 10.1111/ejn.14662
	Sample: Tissue Homogenate

Frontiers in Psychiatry (2019) IF: 3.161

Longitudinal Investigation of Psychotherapy Outcomes (LIPO): Description of the Study Protocol

DOI: 10.3389/fpsyt.2019.00212
	Sample: Serum

NEUROPEPTIDES (2022) IF: 3.286

Altered levels of neurobiological biomarkers at the interface of depression and gestational diabetes mellitus in Asian Indian women

DOI: 10.1016/j.npep.2022.102245
PMID: 35461022	Sample: Plasma

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849

Association of blood lead levels with neurobehavior and BDNF expression in school going children

DOI: 10.1016/j.jtemb.2021.126749
PMID: 33773278	Sample: Serum

PEPTIDES (2022) IF: 3.867

Interleukin-6, undercarboxylated osteocalcin, and brain-derived neurotrophic factor responses to single and repeated sessions of high-intensity interval exercise

DOI: 10.1016/j.peptides.2022.170864
PMID: 36028073	Sample: Plasma

SCHIZOPHRENIA RESEARCH (2018) IF: 3.958

Schizophrenia-associated rs4702 G allele-specific downregulation of FURIN expression by miR-338-3p reduces BDNF production

DOI: 10.1016/j.schres.2018.02.040
	Sample: Human Embryonic Kidney (Hek) 293T Cells

American Journal of Translational Research (2021) IF: 4.06

Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy


PMID: 34650715	Sample: Serum

Stem Cell Research & Therapy (2017) IF: 4.211

Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats

DOI: 10.1186/s13287-017-0731-y
	Sample: Human Periodontal Ligament-Derived Stem Cells (Hpd

EUROPEAN NEUROPSYCHOPHARMACOLOGY (2022) IF: 4.6

The role of BDNF and NGF plasma levels in first-episode schizophrenia: A longitudinal study

DOI: 10.1016/j.euroneuro.2022.02.003
PMID: 35219096	Sample: Plasma

JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791

Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

DOI: 10.1016/j.jpsychires.2022.04.002
PMID: 35429740	Sample: Serum

Journal of Clinical Medicine (2022) IF: 4.964

The Effects of Four Weeks of Chiropractic Spinal Adjustments on Blood Biomarkers in Adults with Chronic Stroke: Secondary Outcomes of a Randomized Controlled Trial

DOI: 10.3390/jcm11247493
	Sample: Serum

Journal of Functional Foods (2022) IF: 5.223

Effects of erinacine A-enriched Hericium erinaceus on elderly hearing-impaired patients: A double-blind, randomized, placebo-controlled clinical trial

DOI: 10.1016/j.jff.2022.105220
	Sample: Serum

Food & Function (2022) IF: 5.396

A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation

DOI: 10.1039/D1FO03896F
	Sample: Serum

Nutrients (2021) IF: 5.719

Effects of 12 Weeks Cosmos caudatus Supplement among Older Adults with Mild Cognitive Impairment: A Randomized, Double-Blind and Placebo-Controlled Trial

DOI: 10.3390/nu13020434
PMID: 33572715	Sample: Serum

Cell Death & Disease (2022) IF: 9.685

LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease

DOI: 10.1038/s41419-022-05068-1
PMID: 35906216	Sample: Plasma

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Reviews/Q&A

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Q&A

A*************ESubmitted [ Jun 07 2024 ]

Asked: We plan to transplant cells expressing human BDNF into B6 mice and measure the amount of human BDNF in the blood. Will the E-EL-H0010 kit detect mouse-derived BDNF?

adminSubmitted [ Jun 07 2024 ]

Answered: Testing of mouse samples is not recommended, as human BDNF may not be well expressed in mice and may be contaminated.And the matrix in the mouse sample may also affect the kit measurement.

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