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Human bFGF/FGF2(Basic Fibroblast Growth Factor) ELISA Kit

Citations(14)Uniprot : P09038

Cat.No.:E-EL-H6042
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human bFGF/FGF2 in samples. No significant cross-reactivity or interference between Human bFGF/FGF2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human bFGF/FGF2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human bFGF/FGF2. You can calculate the concentration of Human bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human bFGF/FGF2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human bFGF/FGF2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	83.31	221.04	991.47	81.61	245.82	870.55
Standard deviation	4.48	10.68	48.09	4.63	11.14	36.39
CV (%)	5.38	4.83	4.85	5.67	4.53	4.18

Recovery

The recovery of Human bFGF/FGF2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-105	100
EDTA plasma (n=8)	87-99	94
Cell culture media (n=8)	87-103	95

Linearity

Samples were spiked with high concentrations of Human bFGF/FGF2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P09038
Synonyms	FGF-2, B-FGF, BFGF, FGFB, HBGF-2, prostatropin, heparin-binding growth factor 2,FGF-β
Research Area	Cancer, Cardiovascular, Developmental biology, Neuroscience, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ACS Applied Materials & Interfaces (2022) IF: 10.383
Artificial Extracellular Matrix Composed of Heparin-Mimicking Polymers for Efficient Anticoagulation and Promotion of Endothelial Cell Proliferation

DOI: 10.1021/acsami.2c13892

PMID: 36302722
Sample: HUVECs
JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
Targeted delivery of fat extract by platelet membrane-cloaked nanocarriers for the treatment of ischemic stroke

DOI: 10.1186/s12951-022-01461-2

PMID: 35642036
Sample: PLGA-FE
CELL PROLIFERATION (2022) IF: 8.7550
Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway

DOI: 10.1111/cpr.13316

PMID: 35869570
Sample: HDFs
BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis

DOI: 10.1016/j.biopha.2023.114289

Sample: cardiac fibroblast
Biomaterials Science (2020) IF: 6.183
A multifunctional shape-adaptive and biodegradable hydrogel with hemorrhage control and broad-spectrum antimicrobial activity for wound healing

DOI: 10.1039/D0BM00800A

Sample: bFGF/OD/EPL-3 hydrogel
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces

DOI: 10.3390/ijms23137070

PMID: 35806083
Sample: hBM-MSCs
Stem Cell Research & Therapy (2022) IF: 6.832
Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder

DOI: 10.1186/s13287-022-02739-w

PMID: 35139904
Sample: Human umbilical endothelial cells (HUVEC)
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
Dual Delivery of bFGF- and NGF-Binding Coacervate Confers Neuroprotection by Promoting Neuronal Proliferation

DOI: 10.1159/000490139

Sample: Serum
Journal of Clinical Medicine (2022) IF: 4.242
Cryopreservation in a Standard Freezer: −28 °C as Alternative Storage Temperature for Amniotic Membrane Transplantation

DOI: 10.3390/jcm11041109

Sample: Human amniotic membrane (hAM)
JOURNAL OF CELLULAR PHYSIOLOGY (2019) IF: 4.522
Ganoderma lucidum polysaccharide reduces melanogenesis by inhibiting the paracrine effects of keratinocytes and fibroblasts via IL‐6/STAT3/FGF2 pathway

DOI: 10.1002/jcp.28844

Sample: HaCaT cells,fibroblasts (FB) cells
MOLECULES (2019) IF: 3.06
Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis

DOI: 10.3390/molecules24183316

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

DOI: 10.1016/j.biopha.2019.108706

PMID: 30844656
Sample: Wound drainage fluid
INTERNATIONAL JOURNAL OF PHOTOENERGY (2021) IF: 2.113
Experimental Study of the Effect of Photobiomodulation Therapy on the Regulation of the Healing Process of Chronic Wounds

DOI: 10.1155/2021/3947895

Sample: serum
PSYCHIATRY RESEARCH (2017) IF: 2.528
Decreased levels of serum fibroblast growth factor-2 in children with autism spectrum disorder

DOI: 10.1016/j.psychres.2017.07.028

Sample: Serum

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