Human CD38 (Cyclic ADP Ribose Hydrolase) ELISA Kit (E-EL-H6308)

Name: Human CD38 (Cyclic ADP Ribose Hydrolase) ELISA Kit
Brand: Elabscience
SKU: E-EL-H6308
Price: 150.0 USD
Availability: InStock

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Human CD38 (Cyclic ADP Ribose Hydrolase) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes CD38 in samples.No significant cross-reactivity or interference between CD38 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.380	2.444	2.412	2.331
1000	1.505	1.511	1.508	1.427
500	0.915	0.923	0.919	0.838
250	0.500	0.524	0.512	0.431
125	0.307	0.317	0.312	0.231
62.5	0.182	0.182	0.182	0.101
31.25	0.127	0.133	0.130	0.049
0	0.084	0.078	0.081	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	98.540	254.340	677.190	103.390	260.680	689.950
Standard deviation	6.840	15.690	38.190	7.620	21.170	48.090
CV（%）	6.940	6.170	5.640	7.370	8.120	6.970

Recovery

The recovery of Human CD38 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	97
EDTA plasma （n=8）	93-106	99
Cell culture media （n=8）	89-102	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CD38 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-105	94-111	96-113
1:2	Average	99	102	103
1:4	Range	89-102	96-109	95-108
1:4	Average	95	103	100
1:8	Range	89-102	94-109	95-109
1:8	Average	95	101	101

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	103.74-113.83
Sample 2（n=16）	89.54-104.07

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD38 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD38 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD38 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CD38 . You can calculate the concentration of CD38 in the samples by comparing the OD of the samples to the standard curve.

Background

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain,muscle,and kidney). In humans,it is expressed at high levels on plasma cells and activated T and B cells,natural killer (NK) lymphocytes,myeloblasts,and erythroblasts. By functioning as both a cyclase and a hydrolase,CD38 mediates lymphocyte activation,adhesion,and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.