Human CD5L (CD5 Antigen-like) ELISA Kit (E-EL-H6293)

Name: Human CD5L (CD5 Antigen-like) ELISA Kit
Brand: Elabscience
SKU: E-EL-H6293
Price: 150.0 USD
Availability: InStock

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Human CD5L (CD5 Antigen-like) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	23.44 pg/mL
Detection Range	39.06-2500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes CD5L in samples.No significant cross-reactivity or interference between CD5L and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2500	2.178	2.206	2.192	2.120
1250	1.592	1.582	1.587	1.515
625	0.832	0.862	0.847	0.775
312.5	0.447	0.437	0.442	0.370
156.25	0.272	0.270	0.271	0.199
78.13	0.165	0.161	0.163	0.091
39.06	0.122	0.122	0.122	0.050
0	0.072	0.072	0.072	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	119.370	336.750	1040.310	121.420	368.400	1117.970
Standard deviation	5.480	15.090	49.310	8.670	29.360	83.070
CV（%）	4.590	4.480	4.740	7.140	7.970	7.430

Recovery

The recovery of Human CD5L was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	92-107	98
Serum （n=8）	91-102	97
EDTA plasma （n=8）	90-104	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CD5L and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-107	90-105	97-110
1:2	Average	101	98	104
1:4	Range	89-104	89-105	99-115
1:4	Average	96	96	107
1:8	Range	92-107	95-110	96-107
1:8	Average	98	100	101

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	85.07-108.77
Sample 2（n=16）	87.60-95.51

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD5L . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD5L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD5L , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CD5L . You can calculate the concentration of CD5L in the samples by comparing the OD of the samples to the standard curve.

Background

CD5L (CD5 molecule-like), also known as API6, PRO229, Spalpha or SP-ALPHA, is a 347 amino acid secreted protein that belongs to the scavenger receptor cysteine-rich (SRCR) family of leukocyte regulating proteins. Expressed in bone marrow, spleen, thymus, lymph node and fetal liver, CD5L is thought to be involved in regulating the immune system via binding to peripheral monocytes and mediating their activation and overall survival. CD5L has three cysteine-rich domains and, in addition to its role in the immune system, may function to inhibit apoptosis and promote macrophage survival.