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Human CEA(Carcinoembryonic Antigen) ELISA Kit

  • Cat.No.:E-EL-H6047

  • Reactivity: Human

To Purchase E-EL-H6047

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T/24
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human CEA in samples. No significant cross-reactivity or interference between Human CEA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human CEA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CEA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CEA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CEA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CEA. You can calculate the concentration of Human CEA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 12 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 12 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CEA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CEA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.49 1.20 4.32 0.50 1.04 4.59
Standard deviation 0.03 0.06 0.18 0.03 0.05 0.19
CV (%) 6.12 5.00 4.17 6.00 4.81 4.14

Recovery

The recovery of Human CEA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-104 96
EDTA plasma (n=8) 93-103 96
Cell culture media (n=8) 92-101 95

Linearity

Samples were spiked with high concentrations of Human CEA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P06731
SynonymsCD66E, CD66, CEACAM5, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Research AreaCancer, Immunology, Stem cells, Tags and Cell Markers

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. TALANTA (2021) IF: 6.057
    Fluorescence-infrared absorption dual-mode nanoprobes based on carbon dots@SiO2 nanorods for ultrasensitive and reliable detection of carcinoembryonic antigen

    DOI: 10.1016/j.talanta.2021.122342

    Sample: Serum
  2. Cancer Biomarkers (2016) IF: 1.736
    Macrophage mannose receptor 1 and S100A9 were identified as serum diagnostic biomarkers for colorectal cancer through a label-free quantitative proteomic analysis

    DOI: 10.3233/CBM-150560

    Sample: Serum
  3. Analytical Methods (2018) IF: 2.073
    A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

    DOI: 10.1039/C8AY02113A

    Sample: Serum
  4. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    Hsa_circ_0124055 and hsa_circ_0101622 regulate proliferation and apoptosis in thyroid cancer and serve as prognostic and diagnostic indicators

    DOI: 10.26355/eurrev_202004_21016

    PMID: 32373972

    Sample: Serum,Tissue Homogenate
  5. ANALYST (2020) IF: 3.978
    In situ amplified QCM immunoassay for carcinoembryonic antigen with colorectal cancer using horseradish peroxidase nanospheres and enzymatic biocatalytic precipitation

    DOI: 10.1039/D0AN01399D

    Sample: Serum
  6. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020) IF: 9.412
    Development of a serum miRNA panel for detection of early stage non-small cell lung cancer

    DOI: 10.1073/pnas.2006212117

    PMID: 32943537

    Sample: Serum
  7. BIOSENSORS & BIOELECTRONICS (2022) IF: 10.618
    Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis

    DOI: 10.1016/j.bios.2022.114487

    PMID: 35780540

    Sample: Exosomes
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