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Human CPP(Copeptin) ELISA Kit

  • Cat.No.:E-EL-H0851

  • Reactivity: Human

To Purchase E-EL-H0851

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes CPP in samples. No significant cross-reactivity or interference between CPP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of CPP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CPP. During the reaction, CPP in the sample or standard competes with a fixed amount of CPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level CPP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level CPP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 105.40 220.10 769.80 112.50 239.30 705.10
Standard deviation 5.50 13.00 34.60 7.20 9.80 25.40
CV (%) 5.22 5.91 4.49 6.40 4.10 3.60


The recovery of CPP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-98 93
EDTA plasma(n=8) 84-96 91
Cell culture media(n=8) 91-105 99


Samples were spiked with high concentrations of Human CPP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01185
SynonymsCT-proAVP, C-Terminal Provasopressin, Vasopressin-Neurophysin 2-Copeptin
Research AreaNeuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


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    DOI: 10.1097/01.hjh.0000522977.62696.64

    Sample: plasma
    The relationship between arginine vasopressin gene polymorphisms and plasma copeptin and hypertensive disorders of pregnancy: a nested case-control study

    DOI: 10.1097/HJH.0000000000003376

    Sample: blood
  3. PLoS One (2021) IF: 3.24
    Copeptin and stress-induced hyperglycemia in critically ill patients: A prospective study

    DOI: 10.1371/journal.pone.0250035

    PMID: 33882083

    Sample: Plasma
  4. Diagnostics (2022) IF: 3.706
    The Role of Circulating Collagen Turnover Biomarkers and Late Gadolinium Enhancement in Patients with Non-Ischemic Dilated Cardiomyopathy

    DOI: 10.3390/diagnostics12061435

    PMID: 35741245

    Sample: serum
  5. Diagnostics (2021) IF: 3.706
    Diagnostic Performance of Serum Biomarkers Fibroblast Growth Factor 21, Galectin-3 and Copeptin for Heart Failure with Preserved Ejection Fraction in a Sample of Patients with Type 2 Diabetes Mellitus

    DOI: 10.3390/diagnostics11091577

    PMID: 34573919

    Sample: serum
  6. Journal of Clinical Hypertension (2018) IF: 2.629
    Acute effects of salt on blood pressure are mediated by serum osmolality

    DOI: 10.1111/jch.13374

    PMID: 30232829

    Sample: Serum
  7. Cardiology Journal (2016) IF: 1.13
    Serum copeptin level can be a helpful biomarker in evaluation of myocardial perfusion scintigraphy results

    DOI: 10.5603/CJ.a2015.0036

    Sample: Serum
  8. Anatolian Journal of Cardiology (2017) IF: 1.19
    Role of sympathetic cotransmitter galanin on autonomic balance in heart failure: an active player or a bystander?

    DOI: 10.14744/AnatolJCardiol.2017.7831

    PMID: 28811392

    Sample: Serum
    Serum copeptin as a biomarker of hypertension in children with obesity

    DOI: 10.1111/ped.15355

    Sample: serum
    Clinical use of copeptin in migraine patients admitted to the emergency department

    DOI: 10.1016/j.ajem.2020.05.009

    Sample: Serum
  11. Clinical Hypertension (2022)
    Assessment of brain natriuretic peptide and copeptin as correlates of blood pressure in chronic hypertensive pregnant women

    DOI: 10.1186/s40885-022-00221-6

    Sample: serum
  12. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2020) IF: 0.373
    Poster Abstracts

    DOI: 10.1515/tjb-2020-45s208

    Sample: blood
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a***********şSubmitted [ Feb 15 2021 ]

Asked: iyi günler 98 T Standart Referans Standardı 2 şişe alındığında toplamda kaç hastada çalışma yapılabiliyor.



adminSubmitted [ Feb 15 2021 ]

Answered: For a 96T kit with 2 vials of standard, Run up to 88 samples if standards and samples are not assayed in duplicate; Run up to 80 samples if only standards are assayed in duplicate; Run up to 44 samples if only samples are assayed in duplicate; Run up to 40 samples if standards and samples are both assayed in duplicate. Moreover, for each sample test, it is necessary to test together with the standards, and use the sub-standard curve to calculate the concentration of the samples.

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