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Human CPP(Copeptin) ELISA Kit

  • Cat.No.:E-EL-H0851

  • Reactivity: Human

To Purchase E-EL-H0851

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes CPP in samples. No significant cross-reactivity or interference between CPP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of CPP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CPP. During the reaction, CPP in the sample or standard competes with a fixed amount of CPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level CPP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level CPP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 105.40 220.10 769.80 112.50 239.30 705.10
Standard deviation 5.50 13.00 34.60 7.20 9.80 25.40
CV (%) 5.22 5.91 4.49 6.40 4.10 3.60

Recovery

The recovery of CPP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-98 93
EDTA plasma(n=8) 84-96 91
Cell culture media(n=8) 91-105 99

Linearity

Samples were spiked with high concentrations of Human CPP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01185
SynonymsCT-proAVP, C-Terminal Provasopressin, Vasopressin-Neurophysin 2-Copeptin

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Hypertension (2017) IF: 4.085
    Copeptin Levels In Patients With Treatment Resistant Hypertension Before And After Renal Denervation.

    DOI: 10.1080/1354750X.2016.1252968

    PMID: 27775435

  2. Anatolian Journal of Cardiology (2017) IF: 1.19
    Role Of sympathetic Cotransmitter Galanin On Autonomic Balance In Heart Failure: An Active Player Or A Bystander.

    DOI: 10.14744/AnatolJCardiol.2017.7831

    PMID: 28811392

    Sample: Serum
  3. Cardiology Journal (2016) IF: 1.256
    Serum Copeptin Level Can Be A Helpful Biomarker In Evaluation Of Myocardial Perfusion Scintigraphy Results.

    DOI: 10.5603/CJ.a2015.0036

    PMID: 26100831

    Sample: Serum
  4. The International Journal of Cardiovascular Imaging (2020)
    Is there an association between sarcoidosis and atherosclerosis?

    DOI: 10.1007/s10554-020-02041-x

    PMID: 32989613

    Sample: Plasma
  5. Bioscience Research (2019)
    Evaluation the role of the hormone copeptin in patients with renal failure

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  6. The American Journal of Emergency Medicine (2020)
    Clinical use of copeptin in migraine patients admitted to the emergency department

    DOI: 10.1016/j.ajem.2020.05.009

    PMID: 99999999

    Sample: Serum
  7. Ko?uyolu Heart Journal (2018)
    Copeptin Level in Isolated Coronary Artery Ectasia

    DOI: 10.5578/khj.66568

    PMID: 99999999

    Sample: Serum
  8. The Journal of Clinical Hypertension (2018)
    Acute effects of salt on blood pressure are mediated by Serum osmolality

    DOI: 10.1111/jch.13374

    PMID: 30232829

    Sample: Serum
  9. The Egyptian Heart Journal (2018) IF: 0.31
    Clinical role of serum Copeptin in acute coronary syndrome.

    DOI: 10.1016/j.ehj.2018.04.008

    PMID: 30190640

    Sample: Serum
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